Nekvapil Tomas, Kopriva Vladimir, Boudny Vladimir, Hostovsky Martin, Dvorak Petr, Malota Ladislav
Department of Biochemistry, Chemistry and Biophysics, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1-3, 61242 Brno, Czech Republic.
ScientificWorldJournal. 2012;2012:361698. doi: 10.1100/2012/361698. Epub 2012 Sep 10.
The aim of this work was to determine antioxidant capacity of beverages containing black, white, and green tea extracts using the photochemiluminescence method, and to monitor its changes based on the storage temperature and time. Samples were stored at two different temperatures (refrigerated at 4°C and laboratory temperature 22°C), analyzed after opening of the original package, and consequently after 4 and 7 days. Results of the antioxidant capacity are expressed as the standard equivalents, that is, ascorbic acid in mmol/L. The highest mean value of the antioxidant capacity was found after opening of the original package in fruit-juice-enriched samples and totaled 9.793 mmol/L. This group revealed significant dependence (P < 0.05) not only on the storage time, but also temperature. In samples without added fruit juices containing preservatives the value was 0.428 mmol/L. This group showed significant dependence (P < 0.05) on the decrease of antioxidant capacity only when based on the storage time. Samples without fruit juices or preservatives showed significant decrease in the antioxidant capacity (P < 0.05) after 4 days of storage based on the storage time. The dependence on temperature was revealed only after 7 days of storage.
本研究旨在采用光化学发光法测定含有红茶、白茶和绿茶提取物的饮料的抗氧化能力,并根据储存温度和时间监测其变化。样品分别储存在两个不同温度下(4℃冷藏和22℃实验室温度),在打开原包装后进行分析,随后在4天和7天后再次分析。抗氧化能力的结果以标准当量表示,即毫摩尔每升的抗坏血酸。在打开原包装后,富含果汁的样品中抗氧化能力的最高平均值为9.793毫摩尔每升。该组不仅在储存时间上,而且在温度上均显示出显著相关性(P<0.05)。在不含添加果汁但含有防腐剂的样品中,该值为0.428毫摩尔每升。该组仅在基于储存时间时,抗氧化能力的下降显示出显著相关性(P<0.05)。不含果汁或防腐剂的样品在储存4天后,基于储存时间其抗氧化能力显著下降(P<0.05)。仅在储存7天后才显示出对温度的相关性。