Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, 14071, Córdoba, Spain.
Environ Microbiol. 2013 Jan;15(1):253-70. doi: 10.1111/j.1462-2920.2012.02875.x. Epub 2012 Sep 24.
Pseudomonas pseudoalcaligenes CECT5344 is a Gram-negative bacterium able to tolerate cyanide and to use it as the sole nitrogen source. We report here the first draft of the whole genome sequence of a P. pseudoalcaligenes strain that assimilates cyanide. Three aspects are specially emphasized in this manuscript. First, some generalities of the genome are shown and discussed in the context of other Pseudomonadaceae genomes, including genome size, G + C content, core genome and singletons among other features. Second, the genome is analysed in the context of cyanide metabolism, describing genes probably involved in cyanide assimilation, like those encoding nitrilases, and genes related to cyanide resistance, like the cio genes encoding the cyanide insensitive oxidases. Finally, the presence of genes probably involved in other processes with a great biotechnological potential like production of bioplastics and biodegradation of pollutants also is discussed.
产碱假单胞菌 CECT5344 是一种能够耐受氰化物并将其用作唯一氮源的革兰氏阴性细菌。本文首次报道了一株能够同化氰化物的产碱假单胞菌菌株的全基因组序列。本文特别强调了三个方面。首先，在其他假单胞菌科基因组的背景下，展示和讨论了基因组的一些概况，包括基因组大小、G+C 含量、核心基因组和单核苷酸多态性等特征。其次，在氰化物代谢的背景下分析了基因组，描述了可能参与氰化物同化的基因，如编码腈酶的基因，以及与氰化物抗性相关的基因，如编码氰化物不敏感氧化酶的 cio 基因。最后，还讨论了可能参与其他具有巨大生物技术潜力的过程的基因的存在，如生物塑料的生产和污染物的生物降解。
