Center for Biotechnology, Bielefeld University, 33615 Bielefeld, Germany.
Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Córdoba, Spain.
J Biotechnol. 2014 Apr 10;175:67-8. doi: 10.1016/j.jbiotec.2014.02.004. Epub 2014 Feb 16.
Pseudomonas pseudoalcaligenes CECT5344, a Gram-negative bacterium isolated from the Guadalquir River (Córdoba, Spain), is able to utilize different cyano-derivatives. Here, the complete genome sequence of P. pseudoalcaligenes CECT5344 harboring a 4,686,340bp circular chromosome encoding 4513 genes and featuring a GC-content of 62.34% is reported. Necessarily, remaining gaps in the genome had to be closed by assembly of few long reads obtained from PacBio single molecule real-time sequencing. Here, the first complete genome sequence for the species P. pseudoalcaligenes is presented.
假单胞菌 CECT5344 是从瓜达尔基维尔河（西班牙科尔多瓦）分离到的革兰氏阴性菌，能够利用不同的氰基衍生物。本文报道了假单胞菌 CECT5344 的全基因组序列，该菌为 4513 个基因组成的 4686340bp 环状染色体，GC 含量为 62.34%。基因组中必然存在一些缺口，需要通过 PacBio 单分子实时测序得到的少数长读段进行组装来填补。本文首次提供了该种假单胞菌的完整基因组序列。
