Burns Unit, Hospital de Pronto Socorro, Porto Alegre, RS, Brazil.
Burns. 2012 Dec;38(8):1143-50. doi: 10.1016/j.burns.2012.07.028. Epub 2012 Sep 19.
Autografts represent the gold standard for the treatment of full thickness burns. Factors such as lack of suitable donor sites and poor skin quality, however, have led to the development of artificial dermal substitutes. The investigation of mechanisms leading to enhanced functionality of these skin substitutes has been attracting great attention. This study aimed to investigate the effect of autologous stem cells on the integration and vascularization of a dermal substitute in full-thickness skin wounds, in a murine model. Two cell populations were compared, whole bone marrow cells and cultivated mesenchymal stem cells, isolated from mice transgenic for the enhanced green fluorescent protein, which allowed tracking of the transplanted cells. The number of cells colonizing the dermal substitute, as well as vascular density, were higher in mice receiving total bone marrow and particularly mesenchymal stem cells, than in control animals. The effect was more pronounced in animals treated with mesenchymal stem cells, which located primarily in the wound bed, suggesting a paracrine therapeutic mechanism. These results indicate that combining mesenchymal stem cells with artificial dermal substitutes may represent an important potential modality for treating full thickness burns, even in allogeneic combinations due to the immunoregulatory property of these cells.
自体移植物是治疗全层烧伤的金标准。然而，由于缺乏合适的供体部位和较差的皮肤质量，导致了人工真皮替代物的发展。对这些皮肤替代物增强功能的机制的研究引起了极大的关注。本研究旨在探讨自体干细胞对全层皮肤伤口中真皮替代物的整合和血管化的影响，建立在一个小鼠模型上。比较了两种细胞群，即来源于转基因增强型绿色荧光蛋白小鼠的全骨髓细胞和培养的间充质干细胞，这允许追踪移植细胞。与对照组动物相比，接受全骨髓和特别是间充质干细胞治疗的小鼠中，定植于真皮替代物的细胞数量和血管密度更高。在接受间充质干细胞治疗的动物中，这种效果更为明显，这些细胞主要位于伤口床中，提示存在旁分泌治疗机制。这些结果表明，将间充质干细胞与人工真皮替代物结合，可能代表一种治疗全层烧伤的重要潜在方法，即使是同种异体组合，也因为这些细胞具有免疫调节特性。
