Shimizu Yusuke, Ntege Edward Hosea, Sunami Hiroshi
Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Nakagami, 903-0215 Okinawa, Japan.
Center for Advanced Medical Research, School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Nakagami, 903-0215 Okinawa, Japan.
Regen Ther. 2022 Jun 15;21:73-80. doi: 10.1016/j.reth.2022.05.008. eCollection 2022 Dec.
Current trends indicate a growing interest among healthcare specialists and the public in the use of regenerative medicine-based approaches for skin regeneration. The approaches are categorised in either cell-based or cell-free therapies and are reportedly safe and effective. Cell-based therapies include mesenchymal stem cells (MSCs), tissue induced pluripotent stem cells (iPSCs), fibroblast-based products, and blood-derived therapies, such as those employing platelet-rich plasma (PRP) products. Cell-free therapies primarily involve the use of MSC-derived extracellular vesicles/exosomes. MSCs are isolated from various tissues, such as fat, bone marrow, umbilical cord, menstrual blood, and foetal skin, and expanded before transplantation. In cell-free therapies, MSC exosomes, MSC-derived cultured media, and MSC-derived extracellular vesicles are collected from MSC-conditioned media or supernatant. In this review, a literature search of the Cochrane Library, MEDLINE (PubMed), EMBASE, and Scopus was conducted using several combinations of terms, such as 'stem', 'cell', 'aging', 'wrinkles', 'nasolabial folds', 'therapy', 'mesenchymal stem cells', and 'skin', to identify relevant articles providing a comprehensive update on the different regenerative medicine-based therapies and their application to skin regeneration. In addition, the regulatory perspectives on the clinical application of some of these therapies in Japan are highlighted.
当前趋势表明，医疗保健专家和公众对使用基于再生医学的方法进行皮肤再生的兴趣日益浓厚。这些方法分为基于细胞的疗法或无细胞疗法，据报道是安全有效的。基于细胞的疗法包括间充质干细胞（MSC）、组织诱导多能干细胞（iPSC）、基于成纤维细胞的产品以及血液衍生疗法，例如那些使用富含血小板血浆（PRP）产品的疗法。无细胞疗法主要涉及使用源自MSC的细胞外囊泡/外泌体。MSC从各种组织中分离出来，如脂肪、骨髓、脐带、月经血和胎儿皮肤，并在移植前进行扩增。在无细胞疗法中，MSC外泌体、源自MSC的培养基和源自MSC的细胞外囊泡是从MSC条件培养基或上清液中收集的。在本综述中，使用了“干细胞”、“细胞”、“衰老”、“皱纹”、“鼻唇沟”、“疗法”、“间充质干细胞”和“皮肤”等术语的几种组合，对Cochrane图书馆、MEDLINE（PubMed）、EMBASE和Scopus进行了文献检索，以识别提供基于再生医学的不同疗法及其在皮肤再生中的应用的全面更新的相关文章。此外，还强调了日本对其中一些疗法临床应用的监管观点。
