Institute of Nutrition, Chinese Academy of Sciences, Shanghai 200031, China.
Neurosci Lett. 2012 Oct 31;529(1):28-32. doi: 10.1016/j.neulet.2012.09.021. Epub 2012 Sep 19.
Intracerebral hemorrhage (ICH), accounting for 15-20% of strokes, can cause significant brain injury and life long neurological deficits. We investigated whether treadmill exercise rehabilitation could improve brain repair after ICH and whether involvement of NFG-TrkA and BDNF-TrkB signaling could be observed during repair period in an experimental mouse ICH model reproduced by heparinized-collagenase infusion into the left caudate putamen. 5-Bromo-2-deoxyuridine (BrdU) labeled new dividing cell can be observed clearly around the injured cortex and striatum region on day 7 (D7) after operation, and both TrkA and TrkB neurotropic receptors were activated. A subgroup of these ICH mice began the treadmill exercise from D4 after operation. Then we found that the overall immunofluorescent signals of p-Y490-TrkA and p-Y705-TrkB were both decreased in all groups at D14 after operation. However, compared to the non-exercise ICH group mouse, the immunofluorescent intensity of BDNF and p-Y705-TrkB were significantly higher in the exercise group. In addition, there was no difference in p-Y490-TrkA. Our results suggest that BDNF-TrkB but not NGF-TrkA signaling is involved in the brain repair after ICH, and early proper treadmill exercise might promote this repair process.
脑出血（ICH）占中风的 15-20%，可导致严重的脑损伤和终身神经功能缺陷。我们研究了跑步机运动康复是否可以改善 ICH 后的大脑修复，以及在肝素化胶原酶注入左侧尾状核-壳核复制的实验性小鼠 ICH 模型的修复期间是否可以观察到 NFG-TrkA 和 BDNF-TrkB 信号的参与。术后第 7 天（D7），可以清楚地观察到损伤皮质和纹状体区域周围有 5-溴-2-脱氧尿苷（BrdU）标记的新分裂细胞，并且 TrkA 和 TrkB 神经营养受体均被激活。一部分 ICH 小鼠在手术后第 4 天开始进行跑步机运动。然后我们发现，与非运动 ICH 组小鼠相比，运动组在术后第 14 天所有组的 p-Y490-TrkA 和 p-Y705-TrkB 的总免疫荧光信号均降低。然而，BDNF 和 p-Y705-TrkB 的免疫荧光强度在运动组中明显高于非运动 ICH 组。此外，p-Y490-TrkA 没有差异。我们的结果表明，BDNF-TrkB 而不是 NGF-TrkA 信号参与了 ICH 后的大脑修复，早期适当的跑步机运动可能会促进这一修复过程。
