Takamatsu Yasuyuki, Tamakoshi Keigo, Waseda Yuya, Ishida Kazuto
Department of Physical Therapy, Program in Physical and Occupational Therapy, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan; Department of Rehabilitation, National Hospital Organization Higashi Nagoya National Hospital, Nagoya, Aichi, Japan.
Department of Physical Therapy, Program in Physical and Occupational Therapy, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan; Department of Physical Therapy, Niigata University of Health and Welfare, Niigata, Japan.
Behav Brain Res. 2016 Mar 1;300:56-64. doi: 10.1016/j.bbr.2015.12.003. Epub 2015 Dec 7.
Rehabilitative approaches benefit motor functional recovery after stroke and relate to neuronal plasticity. We investigated the effects of a treadmill running exercise on the motor functional recovery and neuronal plasticity after collagenase-induced striatal intracerebral hemorrhage (ICH) in rats. Male Wistar rats were injected with type IV collagenase into the left striatum to induce ICH. Sham-operated animals were injected with saline instead of collagenase. The animals were randomly assigned to the sham control (SC), the sham exercise (SE), the ICH control (IC), or the ICH exercise (IE) group. The exercise groups were forced to run on a treadmill at a speed of 9 m/min for 30 min/day between days 4 and 14 after surgery. Behavioral tests were performed using a motor deficit score, a beam-walking test and a cylinder test. At fifteen days after surgery, the animals were sacrificed, and their brains were removed. The motor function of the IE group significantly improved compared with the motor function of the IC group. No significant differences in cortical thickness were found between the groups. The IC group had fewer branches and shorter dendrite lengths compared with the sham groups. However, dendritic branches and lengths were not significantly different between the IE and the other groups. Tropomyosin-related kinase B (TrkB) expression levels increased in the IE compared with IC group, but no significant differences in other protein (brain-derived neurotrophic factor, BDNF; Nogo-A; Rho-A/Rho-associated protein kinase 2, ROCK2) expression levels were found between the groups. These results suggest that improved motor function after a treadmill running exercise after ICH may be related to the prevention of dendritic regression due to TrkB upregulation.
康复方法有利于中风后的运动功能恢复，且与神经元可塑性相关。我们研究了跑步机跑步运动对胶原酶诱导的大鼠纹状体内脑出血（ICH）后运动功能恢复和神经元可塑性的影响。将雄性Wistar大鼠左侧纹状体内注射IV型胶原酶以诱导脑出血。假手术动物注射生理盐水而非胶原酶。将动物随机分为假手术对照组（SC）、假运动组（SE）、ICH对照组（IC）或ICH运动组（IE）。运动组在术后第4天至第14天期间，被迫以9米/分钟的速度在跑步机上跑步30分钟/天。使用运动缺陷评分、横梁行走试验和圆筒试验进行行为测试。术后15天，处死动物并取出大脑。与IC组的运动功能相比，IE组的运动功能显著改善。各组之间皮质厚度未发现显著差异。与假手术组相比，IC组的分支较少且树突长度较短。然而，IE组与其他组之间的树突分支和长度无显著差异。与IC组相比，IE组中肌钙蛋白相关激酶B（TrkB）表达水平升高，但各组之间其他蛋白质（脑源性神经营养因子，BDNF；Nogo-A；Rho-A/Rho相关蛋白激酶2，ROCK2）表达水平未发现显著差异。这些结果表明ICH后跑步机跑步运动后运动功能的改善可能与TrkB上调导致的树突退缩的预防有关。
