Shukla D, Vinuth D P, Sowmya S W, Jeevan M B, Kale A D, Hallikerimath S
Department of Oral Pathology and Microbiology, Faculty of Dentistry, Jamia Millia Islamia, Delhi, India.
J Forensic Odontostomatol. 2012 Jul 1;30(1):29-36.
Dental cementum is a specialized calcified structure covering the root of a tooth. This study aims to investigate cementum using various stains which can be exceedingly useful in investigation, observation and diagnosis. 4µm sections of 25 extracted normal teeth, 25 cases of various cemental pathologies and 25 ground sections were stained using cresyl violet, H/E, toluidine blue and periodic acid Schiff and were observed under light and florescence microscopes. Cresyl violet showed best contrast amongst all stains in decalcified and ground sections under light and florescence microscopy. Under the fluorescence microscope, cementum floresced more distinctly than dentin and enamel. Among the cemental pathologies examined, osteoid and cementoid exhibited florescence but cementum and bone did not fluoresce. Incremental lines were prominently visualised with cresyl violet under fluorescent microscopy, which may aid in forensic determination of age. The present results demonstrate that cementum in normal decalcified teeth and cemento-osseous lesions, could be observed best using cresyl violet stain under florescence microscopy.
牙骨质是覆盖牙齿根部的一种特殊钙化结构。本研究旨在使用多种染色剂对牙骨质进行研究，这些染色剂在研究、观察和诊断中可能非常有用。对25颗拔除的正常牙齿、25例各种牙骨质病变病例的4微米切片以及25个磨片分别用甲酚紫、苏木精/伊红、甲苯胺蓝和过碘酸希夫染色，并在光学显微镜和荧光显微镜下观察。在光学显微镜和荧光显微镜下，甲酚紫在脱钙切片和磨片中的所有染色剂中显示出最佳对比度。在荧光显微镜下，牙骨质的荧光比牙本质和釉质更明显。在所检查的牙骨质病变中，类骨质和类牙骨质呈现荧光，但牙骨质和骨不发荧光。在荧光显微镜下，甲酚紫能清晰地显示出增龄线，这可能有助于法医鉴定年龄。目前的结果表明，在荧光显微镜下，使用甲酚紫染色能最好地观察正常脱钙牙齿和牙骨质骨病变中的牙骨质。
