Daly C G, Seymour G J, Kieser J B, Corbet E F
J Clin Periodontol. 1982 May;9(3):266-74. doi: 10.1111/j.1600-051x.1982.tb02066.x.
Contamination of periodontally involved cementum by bacterial substances such as lipopolysaccharide (LPS) is considered a major reason for root planing. The purpose of the present study was to investigate the presence and location of lipid and polysaccharide within involved cementum as compared with uninvolved cementum. Frozen sections were prepared from the decalcified roots of 36 periodontally diseased and two control teeth. Serial sections were stained for either lipid (Oil-Red-O) or polysaccharide (Alcian Blue - PAS) and also with haematoxylin & eosin (H & E) or Huberstone's gram stain. Specimens of involved and uninvolved cementum were then examined under the light microscope for assessment of differences. Involved cementum from 12 of the periodontally diseased teeth exhibited strongly PAS-positive stained processes penetrating 3-7 mum into the surface of cementum from overlying plaque. Such processes were not observed in uninvolved cementum, suggesting a possible bacterial origin. Lipid granules were noted in only one involved specimen where they were situated up to 10 mum beneath the cemental surface. Similar granules were observed within plaque deposits but never in uninvolved cementum, again suggesting a possible bacterial origin. H & E and gram-stained specimens revealed the presence of microbial deposits in surface defects and within defects at the cemento-dentinal junction (CDJ), as well as penetration of micro-organisms into cementum in the absence of any surface defects. The results indicate that although lipid and polysaccharide of possible bacterial origin may be present within the 10 mum surface zone of involved cementum, the finding of microbial deposits down to the level of the CDJ suggests that all periodontally involved cementum should be removed during root planing, in order to achieve a root surface free of bacterial contamination.
诸如脂多糖（LPS）等细菌物质对牙周受累牙骨质的污染被认为是根面平整的主要原因。本研究的目的是调查与未受累牙骨质相比，受累牙骨质中脂质和多糖的存在情况及位置。从36颗牙周病牙和2颗对照牙的脱钙牙根制备冷冻切片。连续切片分别用脂质（油红O）或多糖（阿尔辛蓝-过碘酸希夫染色）染色，同时还用苏木精和伊红（H&E）或休伯斯通革兰氏染色。然后在光学显微镜下检查受累和未受累牙骨质的标本，以评估差异。12颗牙周病牙的受累牙骨质表现出强烈的过碘酸希夫染色阳性突起，从覆盖的菌斑穿透到牙骨质表面3-7微米深处。在未受累牙骨质中未观察到此类突起，提示可能起源于细菌。仅在一个受累标本中发现脂质颗粒，它们位于牙骨质表面下方达10微米处。在菌斑沉积物中观察到类似颗粒，但在未受累牙骨质中从未观察到，再次提示可能起源于细菌。苏木精和伊红染色及革兰氏染色标本显示，在表面缺陷处以及牙骨质-牙本质交界处（CDJ）的缺陷内存在微生物沉积物，并且在没有任何表面缺陷的情况下微生物侵入牙骨质。结果表明，尽管在受累牙骨质的10微米表面区域内可能存在可能起源于细菌的脂质和多糖，但在CDJ水平发现微生物沉积物表明，在根面平整过程中应去除所有牙周受累的牙骨质，以实现根面无细菌污染。
