Alvarez Y, Borja G, Valladares Y, Alvarez-Noves J, Die R
Cytobios. 1979;24(93):47-65.
The incorporation of mouse S-EAC DNA into homologous normal cells (mouse embryo secondary cultures), and into heterologous cancer cells (TC-SV40 line), with both systems having their native DNA blocked by BrUdR incorporation, was studied. 3H-TdR-DNA was inoculated with DEAE-D to protect it and to potentiate its incorporation, the process being autoradiograohically controlled. The amount of incorporated DNA was radioisotopically determined, and the incorporation process was studied by analysing the fractions obtained after density gradient centrifugation separation of the inoculated cells DNA. Receptivity was greater in those cells inoculated with DEAE-D-protected DNA. The incorporation was slightly greater for cells whose DNA had been blocked by BrUdR incorporation, and for homologous with respect to heterologous cells. In those cells inoculated while the DNA blockade was incomplete, part of the inoculated DNA became incorporated into the cell genome (L-H chains). However, in the completely blocked cells it could not be determined if the incorporation occurred in a lysogenic-like or in an episomic-like form.
研究了将小鼠S-EAC DNA掺入同源正常细胞（小鼠胚胎二次培养物）和异源癌细胞（TC-SV40系）的情况，在这两种系统中，其天然DNA都通过掺入溴脱氧尿苷（BrUdR）而被阻断。用二乙氨基乙基葡聚糖（DEAE-D）接种³H-胸腺嘧啶脱氧核苷（³H-TdR）-DNA以保护它并增强其掺入，该过程通过放射自显影进行控制。通过放射性同位素测定掺入的DNA量，并通过分析接种细胞DNA经密度梯度离心分离后得到的组分来研究掺入过程。接种了DEAE-D保护的DNA的细胞中的接受性更高。对于DNA已被BrUdR掺入阻断的细胞以及同源细胞相对于异源细胞，掺入量略高。在DNA阻断不完全时接种的那些细胞中，部分接种的DNA掺入了细胞基因组（轻链-重链）。然而，在完全阻断的细胞中，无法确定掺入是以溶原样形式还是附加体样形式发生。
