Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH, USA.
Cell Transplant. 2013;22(3):461-8. doi: 10.3727/096368912X656063. Epub 2012 Sep 21.
Mesenchymal stromal cells (MSCs) have differentiation, immunomodulatory, and self-renewal properties and are, therefore, an attractive tool for regenerative medicine and autoimmune diseases. MSCs may be of great value to treat graft-versus-host disease. Influenza virus causes highly contagious seasonal infection and occasional pandemics. The infection is severe in children, elderly, and immunocompromised hosts including hematopoietic stem cell transplant patients. The objective of this study was to determine if MSCs are permissive to influenza virus replication. We isolated MSCs from the bone marrow of 4- to 6-week-old germ-free pigs. Swine and human influenza virus strains were used to infect MSCs in vitro. MSCs expressed known influenza virus α-2,3 and α-2,6 sialic acid receptors and supported replication of swine and human influenza viruses. Viral infection of MSCs resulted in cell lysis and proinflammatory cytokine production. These findings demonstrate that bone marrow-derived MSCs are susceptible to influenza virus. The data also suggest that transplantation of bone marrow MSCs from influenza virus-infected donors may transmit infection to recipients. Also, MSCs may get infected if infused into a patient with an ongoing influenza virus infection.
间充质基质细胞 (MSCs) 具有分化、免疫调节和自我更新的特性，因此是再生医学和自身免疫性疾病的有吸引力的工具。MSCs 可能对治疗移植物抗宿主病具有重要价值。流感病毒引起高度传染性的季节性感染和偶尔的大流行。感染在儿童、老年人和免疫功能低下的宿主中很严重，包括造血干细胞移植患者。本研究的目的是确定 MSCs 是否允许流感病毒复制。我们从 4 至 6 周龄无菌猪的骨髓中分离出 MSCs。使用猪和人流感病毒株在体外感染 MSCs。MSCs 表达已知的流感病毒α-2,3 和 α-2,6 唾液酸受体，并支持猪和人流感病毒的复制。MSCs 的病毒感染导致细胞裂解和促炎细胞因子的产生。这些发现表明骨髓来源的 MSCs 易受流感病毒感染。该数据还表明，来自流感病毒感染供体的骨髓 MSCs 的移植可能会将感染传播给受者。此外，如果将 MSCs 输注到正在发生流感病毒感染的患者中，它们也可能被感染。
