Department of Epidemiology and Public Health, Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Sex Transm Dis. 2012 Oct;39(10):807-12. doi: 10.1097/OLQ.0b013e3182631c79.
Some vaginal bacterial communities are thought to prevent infection by sexually transmitted organisms. Prior work demonstrated that the vaginal microbiota of reproductive-age women cluster into 5 types of bacterial communities; 4 dominated by Lactobacillus species (L. iners, L. crispatus, L. gasseri, L. jensenii) and 1 (termed community state type (CST) IV) lacking significant numbers of lactobacilli and characterized by higher proportions of Atopobium, Prevotella, Parvimonas, Sneathia, Gardnerella, Mobiluncus, and other taxa. We sought to evaluate the relationship between vaginal bacterial composition and Trichomonas vaginalis.
Self-collected vaginal swabs were obtained cross-sectionally from 394 women equally representing 4 ethnic/racial groups. T. vaginalis screening was performed using PCR targeting the 18S rRNA and β-tubulin genes. Vaginal bacterial composition was characterized by pyrosequencing of barcoded 16S rRNA genes. A panel of 11 microsatellite markers was used to genotype T. vaginalis. The association between vaginal microbiota and T. vaginalis was evaluated by exact logistic regression.
T. vaginalis was detected in 2.8% of participants (11/394). Of the 11 T. vaginalis-positive cases, 8 (72%) were categorized as CST-IV, 2 (18%) as communities dominated by L. iners, and 1 (9%) as L. crispatus-dominated (P = 0.05). CST-IV microbiota were associated with an 8-fold increased odds of detecting T. vaginalis compared with women in the L. crispatus-dominated state (OR: 8.26, 95% CI: 1.07-372.65). Seven of the 11 T. vaginalis isolates were assigned to 2 genotypes.
T. vaginalis was associated with vaginal microbiota consisting of low proportions of lactobacilli and high proportions of Mycoplasma, Parvimonas, Sneathia, and other anaerobes.
一些阴道细菌群落被认为可以预防性传播病原体的感染。先前的研究表明，育龄妇女的阴道微生物群集分为 5 种细菌群落类型；其中 4 种主要由乳杆菌（L. iners、L. crispatus、L. gasseri、L. jensenii）组成，1 种（称为社区状态类型（CST）IV）缺乏大量的乳杆菌，其特征是更高比例的 Atopobium、Prevotella、Parvimonas、Sneathia、Gardnerella、Mobiluncus 和其他分类群。我们试图评估阴道细菌组成与阴道毛滴虫之间的关系。
从 394 名代表 4 个种族/族群的女性中，采用横截面自我采集的阴道拭子进行研究。采用针对 18S rRNA 和 β-微管蛋白基因的 PCR 对阴道毛滴虫进行筛查。通过对带有条形码的 16S rRNA 基因进行 pyrosequencing 来描述阴道细菌组成。使用 11 个微卫星标记物对阴道毛滴虫进行基因分型。采用精确的逻辑回归评估阴道微生物群与阴道毛滴虫之间的关联。
在 394 名参与者中，有 2.8%（11/394）检测到阴道毛滴虫。在 11 例阴道毛滴虫阳性病例中，8 例（72%）为 CST-IV 型，2 例（18%）为 L. iners 主导型，1 例（9%）为 L. crispatus 主导型（P=0.05）。与处于 L. crispatus 主导状态的女性相比，CST-IV 微生物群与检测到阴道毛滴虫的几率增加 8 倍（OR：8.26，95%CI：1.07-372.65）。11 例阴道毛滴虫分离株中的 7 株被分配到 2 种基因型。
阴道毛滴虫与阴道微生物群相关，其特征是乳杆菌比例较低，而支原体、Parvimonas、Sneathia 和其他厌氧菌比例较高。
