Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, People's Republic of China.
Appl Microbiol Biotechnol. 2013 Jul;97(13):6051-5. doi: 10.1007/s00253-012-4422-4. Epub 2012 Sep 25.
The biological treatment of triphenylmethane dyes is an important issue. Most microbes have limited practical application because they cannot completely detoxicate these dyes. In this study, the extractive biodecolorization of triphenylmethane dyes by Aeromonas hydrophila DN322p was carried out by introducing the cloud point system. The cloud point system is composed of a mixture of nonionic surfactants (20 g/L) Brij 30 and Tergitol TMN-3 in equal proportions. After the decolorization of crystal violet, a higher wet cell weight was obtained in the cloud point system than that of the control system. Based on the results of thin-layer chromatography, the residual crystal violet and its decolorized product, leuco crystal violet, preferred to partition into the coacervate phase. Therefore, the detoxification of the dilute phase was achieved, which indicated that the dilute phase could be discharged without causing dye pollution. The extractive biodecolorization of three other triphenylmethane dyes was also examined in this system. The decolorization of malachite green and brilliant green was similar to that of crystal violet. Only ethyl violet achieved a poor decolorization rate because DN322p decolorized it via adsorption but did not convert it into its leuco form. This study provides potential application of biological treatment in triphenylmethane dye wastewater.
三苯甲烷染料的生物处理是一个重要的问题。大多数微生物由于不能完全解毒这些染料，因此实际应用有限。在这项研究中，通过引入浊点体系，利用嗜水气单胞菌 DN322p 对三苯甲烷染料进行萃取生物脱色。浊点体系由等比例混合的非离子表面活性剂（20 g/L）Brij 30 和 Tergitol TMN-3 组成。在对结晶紫进行脱色后，浊点体系中的湿细胞重量比对照组更高。基于薄层层析的结果，残余的结晶紫及其褪色产物隐色结晶紫更倾向于分配到凝聚相。因此，实现了稀释相的解毒，表明可以排出稀释相而不会造成染料污染。在该体系中还考察了另外三种三苯甲烷染料的萃取生物脱色。孔雀石绿和灿烂绿的脱色与结晶紫相似。只有乙基紫的脱色率较差，因为 DN322p 通过吸附对其进行脱色，但没有将其转化为隐色形式。本研究为三苯甲烷染料废水的生物处理提供了潜在的应用。
