Cheriaa Jihane, Khaireddine Monia, Rouabhia Mahmoud, Bakhrouf Amina
Laboratory of Analysis, Treatment, Valorisation and Environmental Pollution and Products, Faculty of Pharmacy, University of Monastir, Monastir Avicenne Street, Monastir 5000, Tunisia.
ScientificWorldJournal. 2012;2012:512454. doi: 10.1100/2012/512454. Epub 2012 May 1.
A new consortium of four bacterial isolates (Agrobacterium radiobacter; Bacillus spp.; Sphingomonas paucimobilis, and Aeromonas hydrophila)-(CM-4) was used to degrade and to decolorize triphenylmethane dyes. All bacteria were isolated from activated sludge extracted from a wastewater treatment station of a dyeing industry plant. Individual bacterial isolates exhibited a remarkable color-removal capability against crystal violet (50 mg/L) and malachite green (50 mg/L) dyes within 24 h. Interestingly, the microbial consortium CM-4 shows a high decolorizing percentage for crystal violet and malachite green, respectively, 91% and 99% within 2 h. The rate of chemical oxygen demand (COD) removal increases after 24 h, reaching 61.5% and 84.2% for crystal violet and malachite green, respectively. UV-Visible absorption spectra, FTIR analysis and the inspection of bacterial cells growth indicated that color removal by the CM-4 was due to biodegradation. Evaluation of mutagenicity by using Salmonella typhimurium test strains, TA98 and TA100 studies revealed that the degradation of crystal violet and malachite green by CM-4 did not lead to mutagenic products. Altogether, these results demonstrated the usefulness of the bacterial consortium in the treatment of the textile dyes.
一个由四种细菌分离株（放射形土壤杆菌、芽孢杆菌属、少动鞘氨醇单胞菌和气单胞菌）组成的新联合体（CM-4）被用于降解和使三苯甲烷染料脱色。所有细菌均从一家印染厂废水处理站提取的活性污泥中分离得到。单个细菌分离株在24小时内对结晶紫（50毫克/升）和孔雀石绿（50毫克/升）染料表现出显著的脱色能力。有趣的是，微生物联合体CM-4对结晶紫和孔雀石绿分别在2小时内显示出91%和99%的高脱色率。24小时后化学需氧量（COD）的去除率增加，结晶紫和孔雀石绿分别达到61.5%和84.2%。紫外可见吸收光谱、傅里叶变换红外光谱分析以及细菌细胞生长检查表明，CM-4的脱色是由于生物降解。使用鼠伤寒沙门氏菌测试菌株TA98和TA100进行的致突变性评估研究表明，CM-4对结晶紫和孔雀石绿的降解不会产生致突变产物。总之，这些结果证明了该细菌联合体在处理纺织染料方面的有效性。
