Wu Jing, Jung Byung-Gil, Kim Kyoung-Sook, Lee Young-Choon, Sung Nak-Chang
Department of Biotechnology, Dong-A University, Busan 604-714, South Korea.
J Environ Sci (China). 2009;21(7):960-4. doi: 10.1016/s1001-0742(08)62368-2.
Pseudomonas otitidis WL-13, which has a high capacity to decolorize triphenylmethane dyes, was isolated from activated sludge obtained from a wastewater treatment plant of a dyeing industry. This strain exhibited a remarkable color-removal capability when tested against several triphenylmethane dyes under both shaking and static conditions at high concentrations of dyes. More than 95% of Malachite Green and Brilliant Green was removed within 12 h at 500 micromol/L dye concentration under shaking conditions. Crystal Violet lost about 13% of its color under the same conditions tested. The rate of decolorization increased when the M9 medium was supplemented with yeast extract. The optimum pH and temperature for color removal were 7-9 and 35-40 degrees C, respectively. The observed changes in the visible spectra and the inspection of bacterial growth indicated the color-removal by the adsorption of dye to the cells during incubation with strains.
从一家印染行业污水处理厂的活性污泥中分离出了具有高脱色三苯甲烷染料能力的耳炎假单胞菌WL-13。在高浓度染料的振荡和静态条件下，用几种三苯甲烷染料对该菌株进行测试时，它表现出了显著的脱色能力。在振荡条件下，当染料浓度为500微摩尔/升时，12小时内孔雀石绿和亮绿的去除率超过95%。在相同测试条件下，结晶紫的颜色损失约13%。当M9培养基中添加酵母提取物时，脱色率增加。脱色的最佳pH值和温度分别为7-9和35-40摄氏度。可见光谱的观察变化和细菌生长检查表明，在与菌株培养期间，染料通过吸附到细胞上而被脱色。
