Reitan J A, Kien N D, Thorup S, Corkill G
Department of Anesthesiology, University of California School of Medicine, Davis 95616.
Stroke. 1990 Jan;21(1):119-23. doi: 10.1161/01.str.21.1.119.
We studied the effects of graded exposure to hyperbaric (1,875 mm Hg) oxygen therapy in an acute stroke model prepared by unilateral carotid artery interruption in gerbils. Pentobarbital alone, superoxide dismutase alone, two periods of hyperbaric oxygen alone, and each agent combined with hyperbaric oxygen were administered to investigate possible mechanisms of protection from cerebral ischemia. Survival rates and neurologic deficit scores over 5 days in all treated groups were compared with those in a control group. Survival rates in the groups subjected to 2 (63.9 +/- 4.0%) and 4 hours (70.1 +/- 5.2%) of hyperbaric oxygen alone were significantly higher than in the control group (53.6 +/- 4.2%). The group treated with pentobarbital alone also demonstrated increased survival (69.8 +/- 7.0%), but the combination of therapeutic regimens offered no apparent additive protection. By 5 days there were no differences in the neurologic deficit scores of the survivors in the groups. The toxic pulmonary effects of hyperbaric oxygen were assessed in a pilot LD50 study. The pressure used caused no mortality during 4 hours of exposure, and the calculated LD50 was 7.26 hours. This investigation demonstrates that graded doses of hyperbaric oxygen given after the insult increase survival in a gerbil model of stroke.
我们在通过单侧颈动脉阻断制备的沙鼠急性脑卒中模型中，研究了分级暴露于高压（1875毫米汞柱）氧疗的效果。单独给予戊巴比妥、单独给予超氧化物歧化酶、单独给予两个疗程的高压氧，以及每种药物与高压氧联合使用，以研究预防脑缺血的可能保护机制。将所有治疗组5天内的生存率和神经功能缺损评分与对照组进行比较。单独接受2小时（63.9±4.0%）和4小时（70.1±5.2%）高压氧治疗的组的生存率显著高于对照组（53.6±4.2%）。单独用戊巴比妥治疗的组也显示出生存率增加（69.8±7.0%），但联合治疗方案未提供明显的附加保护。到第5天，各存活组的神经功能缺损评分没有差异。在一项初步的半数致死量（LD50）研究中评估了高压氧的毒性肺效应。所用压力在4小时暴露期间未导致死亡，计算出的LD50为7.26小时。这项研究表明，损伤后给予分级剂量的高压氧可提高沙鼠脑卒中模型的生存率。
