Department of Chemistry, University of Pune, Pune 411007, India.
Colloids Surf B Biointerfaces. 2013 Feb 1;102:273-80. doi: 10.1016/j.colsurfb.2012.08.030. Epub 2012 Aug 24.
Nanosized (8-9 nm) Nd doped and Ag coated TiO(2) nanoparticles have been synthesized by sol-gel method. The physicochemical properties of these particles were investigated by X-ray diffraction (XRD), diffuse reflectance UV-visible (DRUV) spectra and Brunauer-Emmett-Teller (BET) surface area analysis. The antibacterial activities of the samples were studied for Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) both, under the light and dark conditions. The results reveal that the extent of activity shows the order as undoped TiO(2)<Nd doped TiO(2)<Ag coated TiO(2). The mechanism of bactericidal action of the nanoparticles, in presence of sunlight has been explained with the help of microscopic analyses. The bacterial damage is observed to proceed through initial perforation of the cell, damage of cell wall and finally the bacterial death.
通过溶胶-凝胶法合成了纳米级（8-9nm）掺钕和镀银的 TiO(2)纳米粒子。通过 X 射线衍射（XRD）、漫反射紫外-可见（DRUV）光谱和 Brunauer-Emmett-Teller（BET）表面积分析研究了这些粒子的物理化学性质。在光照和黑暗条件下，研究了样品对金黄色葡萄球菌（S. aureus）和大肠杆菌（E. coli）的抗菌活性。结果表明，活性程度的顺序为未掺杂 TiO(2)<Nd 掺杂 TiO(2)<Ag 包覆 TiO(2)。借助显微镜分析，解释了纳米粒子在阳光照射下的杀菌作用机制。通过对细胞的初始穿孔、细胞壁的损伤以及最终细菌的死亡，观察到细菌的损伤过程。
