Department of Reproduction and Artificial Insemination, Veterinary Faculty, Selçuk University, Konya, Turkey.
Cryobiology. 2012 Dec;65(3):335-7. doi: 10.1016/j.cryobiol.2012.09.002. Epub 2012 Sep 23.
The aim of this study was to investigate the effects of methionine and lipoic acid on ram sperm parameters during liquid storage (5 °C). Ejaculates collected from five Merino rams were pooled at 37 °C. Each pooled ejaculate was divided into five equal aliquots and diluted (37 °C) with five extenders, one of which was without additives, two of which contained methionine at two different doses, and the other two of which contained lipoic acid at two different doses. Sperm parameters were determined at 0, 24, 48, 72 and 96 h of liquid storage at 5 °C. The extenders containing 2 and 4mM of methionine resulted in higher motility percentages, in comparison to the control, up to 96 h of storage. Methionine at doses of 2 and 4mM led to higher viability and sperm mitochondrial activity percentages, when compared to the controls during 48, 72 and 96 h of liquid storage (P<0.05). The findings of this study showed that methionine was of greater benefit to ram sperm parameters during liquid storage.
本研究旨在探讨蛋氨酸和硫辛酸对绵羊精子参数在液态储存（5°C）期间的影响。从五只美利奴羊采集的精液在 37°C 下收集并混合。每个混合精液等分五份，并与五种稀释液（37°C）混合，其中一种不含添加剂，两种含有两种不同剂量的蛋氨酸，另外两种含有两种不同剂量的硫辛酸。在 5°C 液态储存 0、24、48、72 和 96 小时后测定精子参数。与对照组相比，含有 2 和 4mM 蛋氨酸的稀释液在 96 小时的储存期内具有更高的精子运动百分比。在 48、72 和 96 小时的液态储存期间，2 和 4mM 剂量的蛋氨酸与对照组相比，具有更高的精子活力和精子线粒体活性百分比（P<0.05）。本研究结果表明，蛋氨酸在液态储存期间对绵羊精子参数更有益。
