Institut für Medizinische Mikrobiologie und Immunologie der Universität Bonn, Sigmund-Freud-Straße 25, 53127 Bonn, Germany.
Syst Appl Microbiol. 2012 Oct;35(7):421-6. doi: 10.1016/j.syapm.2012.08.003. Epub 2012 Sep 23.
A Gram-reaction-negative bacterium, strain IMMIB L-12475(T), was isolated from blood cultures of a human with septicaemia. The yellowish orange pigmented strain contained flexirubin pigment. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain IMMIB L-12475(T) belonged to the family Flavobacteriaceae, forming a distinct phyletic line that is distantly related (79.1-89.4% sequence similarity) to described genera of this family. Membership to the family was confirmed by a fatty acid profile consisting of branched-chain and 3-hydroxy fatty acids with major amounts of iso-C(17:0) 3-OH and iso-C(15:0), by the presence of menaquinone MK-6 as the only respiratory quinone and a polyamine pattern that contained sym-homospermidine as major component. The phospholipids consisted of phosphatidylethanolamine and an unknown phospholipid. The genomic DNA mol% G+C content was 45.6%. The distant phylogenetic position as compared to other representative of the family and the significant phenotypic properties such as pigment composition, morphology, and physiology support the proposal of a novel genus and species Cruoricaptor ignavus gen. nov., sp. nov. The type strain is IMMIB L-12475(T) (=DSM 25479(T)=CCUG 62025(T)).
一株革兰氏反应阴性细菌，菌株 IMMIB L-12475(T)，从败血症患者的血培养物中分离得到。该呈黄橙色的色素菌株含有柔韧菌素色素。基于 16S rRNA 基因序列的系统发育分析表明，菌株 IMMIB L-12475(T)属于黄杆菌科，形成一个独特的进化枝，与该科中描述的属有较远的亲缘关系（79.1-89.4%序列相似性）。通过脂肪酸图谱、menaquinone MK-6 作为唯一的呼吸醌和聚胺模式（包含主要成分 sym-homospermidine）证实了该家族的成员身份，脂肪酸图谱由支链和 3-羟基脂肪酸组成，主要含有 iso-C(17:0) 3-OH 和 iso-C(15:0)。磷脂由磷脂酰乙醇胺和一种未知的磷脂组成。基因组 DNA mol% G+C 含量为 45.6%。与该科其他代表菌株相比，其遥远的系统发育位置以及显著的表型特性，如色素组成、形态和生理特性，支持了一个新属和新种 Cruoricaptor ignavus gen. nov.，sp. nov.的提议。该模式菌株为 IMMIB L-12475(T)（=DSM 25479(T)=CCUG 62025(T)）。
