皮肤鳞状细胞癌和角化棘皮瘤中的DNA含量及增殖指数

DNA content and proliferative index in cutaneous squamous cell carcinoma and keratoacanthoma.

作者信息

Randall M B, Geisinger K R, Kute T E, Buss D H, Prichard R W

机构信息

Department of Pathology, Wake Forest University, Bowman Gray School of Medicine, Winston-Salem, North Carolina 27103.

出版信息

Am J Clin Pathol. 1990 Feb;93(2):259-62. doi: 10.1093/ajcp/93.2.259.

DOI:10.1093/ajcp/93.2.259

PMID:2301286

Abstract

The histologic separation of keratoacanthomas (KA) and well-differentiated squamous cell carcinoma (WDSCC) using established criteria may present a diagnostic dilemma in the individual case. The authors questioned whether the DNA index (DI) and/or the proliferative index (PI), as shown by flow cytometry (FCM) might assist in this differential diagnosis. Thirty-six well-differentiated squamous cell lesions of skin were independently classified as either WDSCC or KA by a panel of three pathologists. Six poorly differentiated squamous cell carcinomas (PDSCC) also were included in this study. Sections from paraffin blocks were prepared by standard techniques and analyzed by FCM. Mean DI values were: KA 0.96%, WDSCC 0.99%, and PDSCC 0.88%. The differences in the mean DIs were not statistically significant. Mean PI values were as follows: KA 16.7%, WDSCC 14.8%, and PDSCC 20.2%. Differences were not statistically significant. The authors conclude that the FCM measurements of DI and PI do not help in separating KA and WDSCC of skin.

摘要

运用既定标准对角化棘皮瘤（KA）和高分化鳞状细胞癌（WDSCC）进行组织学区分，在个别病例中可能会带来诊断难题。作者质疑，如流式细胞术（FCM）所示的DNA指数（DI）和/或增殖指数（PI）是否有助于这种鉴别诊断。由三名病理学家组成的小组将36例皮肤高分化鳞状细胞病变独立分类为WDSCC或KA。本研究还纳入了6例低分化鳞状细胞癌（PDSCC）。通过标准技术制备石蜡块切片，并进行FCM分析。平均DI值分别为：KA 0.96%，WDSCC 0.99%，PDSCC 0.88%。平均DI值的差异无统计学意义。平均PI值如下：KA 16.7%，WDSCC 14.8%，PDSCC 20.2%。差异无统计学意义。作者得出结论，FCM对DI和PI的测量无助于区分皮肤的KA和WDSCC。