Steinke John W, Payne Spencer C, Chen Philip G, Negri Julie, Stelow Edward B, Borish Larry
Asthma and Allergic Disease Center, Carter Immunology Center, Department of Medicine, University of Virginia Health Systems, Charlottesville, Virginia, USA.
Ann Otol Rhinol Laryngol. 2012 Sep;121(9):579-86. doi: 10.1177/000348941212100904.
The objective was to determine whether the polyp subtypes observed in cystic fibrosis (CF)-related sinusitis were similar to those observed in non-CF-related sinusitis.
Polyp and mucus samples were collected from CF patients who presented for sinus surgery. The polyps underwent histologic and cytochemical evaluation for the presence of lymphocyte cell populations and their respective cytokine markers. The mucus samples were evaluated for DNA content.
Of the polyps, 42% had an eosinophilic infiltrate, of which 80% had an additional mixed neutrophilic infiltrate. Of the remaining polyp samples, 42% did not have a granulocytic infiltrate, consistent with non-eosinophilic polyps. All samples had CD138-positive plasma cells. The mucus samples from the patients with CF showed higher extracellular DNA concentrations than did the mucus samples from patients with non-CF sinus disease.
Cystic fibrosis-related polyps demonstrated an eosinophil-based dichotomy similar to that of idiopathic non-CF-related polyps. Many also demonstrated neutrophilic infiltrate, indicating that chronic mucus stasis and infection complicate the disease. Agents capable of reducing extracellular DNA may help manage sinusitis in CF patients.
确定在囊性纤维化（CF）相关性鼻窦炎中观察到的息肉亚型是否与非CF相关性鼻窦炎中观察到的息肉亚型相似。
从因鼻窦手术就诊的CF患者中收集息肉和黏液样本。对息肉进行组织学和细胞化学评估，以确定淋巴细胞群体及其各自细胞因子标志物的存在情况。对黏液样本进行DNA含量评估。
在息肉中，42%有嗜酸性粒细胞浸润，其中80%还有额外的混合性中性粒细胞浸润。在其余的息肉样本中，42%没有粒细胞浸润，符合非嗜酸性息肉的特征。所有样本均有CD138阳性浆细胞。CF患者的黏液样本显示细胞外DNA浓度高于非CF鼻窦疾病患者的黏液样本。
CF相关性息肉表现出与特发性非CF相关性息肉相似的基于嗜酸性粒细胞的二分法。许多息肉还表现出中性粒细胞浸润，表明慢性黏液淤滞和感染使病情复杂化。能够降低细胞外DNA的药物可能有助于治疗CF患者的鼻窦炎。
