Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture CENA, University of São Paulo USP, Piracicaba, SP, Brazil.
FEMS Microbiol Ecol. 2013 Mar;83(3):607-21. doi: 10.1111/1574-6941.12018. Epub 2012 Oct 19.
This study focused on the impact of land-use changes and agricultural management of soybean in Amazon forest soils on the abundance and composition of the acidobacterial community. Quantitative real-time PCR (q-PCR) assays and pyrosequencing of 16S rRNA gene were applied to study the acidobacterial community in bulk soil samples from soybean croplands and adjacent native forests, and mesocosm soil samples from soybean rhizosphere. Based on qPCR measurements, Acidobacteria accounted for 23% in forest soils, 18% in cropland soils, and 14% in soybean rhizosphere of the total bacterial signals. From the 16S rRNA gene sequences of Bacteria domain, the phylum Acidobacteria represented 28% of the sequences from forest soils, 16% from cropland soils, and 17% from soybean rhizosphere. Acidobacteria subgroups 1-8, 10, 11, 13, 17, 18, 22, and 25 were detected with subgroup 1 as dominant among them. Subgroups 4, 6, and 7 were significantly higher in cropland soils than in forest soils, which subgroups responded to decrease in soil aluminum. Subgroups 6 and 7 responded to high content of soil Ca, Mg, Mn, and B. These results showed a differential response of the Acidobacteria subgroups to abiotic soil factors, and open the possibilities to explore acidobacterial subgroups as early-warning bioindicators of agricultural soil management effects in the Amazon area.
本研究关注的是亚马逊森林土壤中土地利用变化和大豆农业管理对酸杆菌群落丰度和组成的影响。采用定量实时 PCR（q-PCR）检测和 16S rRNA 基因焦磷酸测序研究了大豆农田和相邻原生林土壤的土壤样本和大豆根际土壤样本中的酸杆菌群落。基于 qPCR 测量，在森林土壤中，酸杆菌占总细菌信号的 23%，在农田土壤中占 18%，在大豆根际中占 14%。从细菌域的 16S rRNA 基因序列来看，在森林土壤中，酸杆菌门代表了 28%的序列，在农田土壤中占 16%，在大豆根际中占 17%。检测到酸杆菌亚群 1-8、10、11、13、17、18、22 和 25，其中亚群 1 占优势。与森林土壤相比，农田土壤中的亚群 4、6 和 7 含量显著更高，这些亚群对土壤铝含量降低有反应。亚群 6 和 7 对土壤 Ca、Mg、Mn 和 B 的含量较高有反应。这些结果表明酸杆菌亚群对非生物土壤因素有不同的反应，为探索酸杆菌亚群作为亚马逊地区农业土壤管理效果的早期预警生物指标提供了可能性。
