日均增重、粪便干物质含量和粪便中细胞内罗森西亚菌浓度之间的关系。
Association between average daily gain, faecal dry matter content and concentration of Lawsonia intracellularis in faeces.
机构信息
Department of Large Animal Sciences, HERD-Centre for Herd oriented Education, Research and Development, University of Copenhagen, Groennegaardsvej 2, Frederiksberg C 1870, Denmark.
出版信息
Acta Vet Scand. 2012 Sep 26;54(1):58. doi: 10.1186/1751-0147-54-58.
BACKGROUND
The objective of this study was to investigate the association between average daily gain and the number of Lawsonia intracellularis bacteria in faeces of growing pigs with different levels of diarrhoea.
METHODS
A longitudinal field study (n = 150 pigs) was performed in a Danish herd from day 29 to 47 post weaning. Every third day all pigs were weighed, subjected to a clinical examination and faecal samples were obtained. Faecal samples were subjected to dry matter determination and absolute quantification by PCR for L. intracellularis and porcine circovirus type 2 (PCV2). Association between average daily gain, faecal dry matter content, numbers of L. intracellularis bacteria and PCV2 genome copies in faeces was investigated in a multilevel mixed-effects linear model.
RESULTS
Increasing numbers of L. intracellularis log10 bacteria/g faeces were significantly associated with decreasing average daily gain (P < 0.001). The association was decreasing with increasing faecal dry matter content (P < 0.01). The number of PCV2 log10 copies/g faeces was not significantly associated with average daily gain of the pigs (P > 0.5).
CONCLUSION
The results suggest a potential application of a PCR quantifying L. intracellularis in growing pigs. Faecal dry matter content must be taken into consideration in interpretation of such test results.
背景
本研究旨在探讨不同腹泻水平生长猪粪便中平均日增重与肠细胞内劳森菌数量的关系。
方法
在丹麦的一个猪群中进行了一项纵向现场研究（n=150 头猪），从断奶后第 29 天到第 47 天进行。每隔三天对所有猪进行称重、临床检查和采集粪便样本。粪便样本进行干物质测定和实时 PCR 定量检测肠细胞内劳森菌和猪圆环病毒 2（PCV2）。在多水平混合效应线性模型中，研究了平均日增重、粪便干物质含量、肠细胞内劳森菌数量和粪便中 PCV2 基因组拷贝数之间的关系。
结果
粪便中肠细胞内劳森菌对数 10 细菌/g 的数量与平均日增重呈显著负相关（P<0.001）。这种关联随着粪便干物质含量的增加而减少（P<0.01）。粪便中 PCV2 对数 10 拷贝/g 的数量与猪的平均日增重无显著相关性（P>0.5）。
结论
结果表明，PCR 定量检测生长猪肠细胞内劳森菌可能有一定的应用。在解释这些检测结果时，必须考虑粪便干物质含量。
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