高危儿童中变形链球菌母婴传播相关因素的初步研究

Factors related to maternal transmission of mutans streptococci in high-risk children-pilot study.

作者信息

Zhan Ling, Tan Stella, Den Besten Pamela, Featherstone John D B, Hoover Charles I

机构信息

Department of Preventive and Restorative Dental Sciences, the University of California, San Francisco, Calif, USA.

出版信息

Pediatr Dent. 2012 Jul-Aug;34(4):e86-91.

PMID:23014079

Abstract

PURPOSE

The purpose of this study was to investigate virulence factors associated with maternal transmission of mutans streptococci (MS).

METHODS

Saliva samples were collected from 10 mothers with active caries and their 2- to 5-year-old children. Ten MS colonies were isolated from each subject. Transmission of MS was identified by arbitrarily primed polymerase chain reactions. Biofilm formation and mutacin production of the isolates against Streptococcus gordonii 10558, Streptococcus sanguinis 10557, Streptococcus mutans 25175, and Streptococcus sobrinus 6715 were analyzed.

RESULTS

All mothers and children had MS colonization. Only 7 of the 36 maternal genotypes (33 Streptococcus mutans genotypes and 3 Streptococcus sobrinus genotypes) were transmitted. Maternal transmission was found in 4 mother-child pairs, whereas 9 children had nonmaternal genotypes. There was no difference in biofilm formation between transmitted and nontransmitted genotypes (P>.05). Transmitted genotypes, however, produced more mutacin against Streptococcus sobrinus 6715 than nontransmitted genotypes.

CONCLUSIONS

This pilot study showed that there may be nonmaternal as well as maternal mutans streptococci transmission.

摘要

目的

本研究旨在调查与变形链球菌（MS）母婴传播相关的毒力因子。

方法

收集10名患有活动性龋齿的母亲及其2至5岁子女的唾液样本。从每个受试者中分离出10个MS菌落。通过任意引物聚合酶链反应鉴定MS的传播情况。分析分离株对戈登链球菌10558、血链球菌10557、变形链球菌25175和远缘链球菌6715的生物膜形成和变链菌素产生情况。

结果

所有母亲和儿童均有MS定植。36种母亲基因型（33种变形链球菌基因型和3种远缘链球菌基因型）中只有7种发生了传播。在4对母婴中发现了母婴传播，而9名儿童具有非母亲基因型。传播基因型和非传播基因型之间的生物膜形成没有差异（P>0.05）。然而，传播基因型对远缘链球菌6715产生的变链菌素比非传播基因型更多。