槲皮素和山柰酚对变异链球菌生物膜活性的影响。
Activity of quercetin and kaemferol against Streptococcus mutans biofilm.
机构信息
Eastman Institute for Oral Health, University of Rochester Medical Center, Rochester, NY, 14618, USA; Department of Forensic Medicine, North Sichuan Medical College, China.
Eastman Institute for Oral Health, University of Rochester Medical Center, Rochester, NY, 14618, USA.
出版信息
Arch Oral Biol. 2019 Feb;98:9-16. doi: 10.1016/j.archoralbio.2018.11.005. Epub 2018 Nov 2.
OBJECTIVE
Nidus Vespae (NV) is the honeycomb of Polistes Olivaceous, P. Japonicus Saussure, and Parapolybiavaria Fabricius. Previously, we have shown the extract and chemical fractions from NV demonstrated remarkable capacities of inhibiting the acid production of oral bacteria at sub-minimum inhibitory concentration (MIC) concentrations. In searching the most potent anti-caries compounds in NV, we further separated the NV Chl/MeOH fraction and obtained two purified compounds: quercetin and kaemferol. The objective of this study was to assess the effectiveness of quercetin and kaemferol against S. mutans biofilm formation.
METHODS
The MIC, minimum biofilm inhibition concentration (MBIC) and minimum biofilm reduction concentration (MBRC) against Streptococcus mutans were examined for NV-derived of quercetin and kaemferol. The effectiveness of inhibiting S. mutans biofilm formation was further examined using in vitro biofilm model.
RESULTS
Both quercetin and kaemferol compounds demonstrated anti-biofilm activities when compared to the negative control. They are capable of reducing biofilm dry-weight, total protein, viable cells measured by colony forming unit (CFU), insoluble and soluble glucans formation. The in situ culture pH was less acidic when the biofilms were treated by quercetin and kaemferol. The quercetin and kaemferol demonstrated comparable capability of S. mutans killing in biofilms, compared to chlorhexidine.
CONCLUSIONS
The results of this study showed inhibitory activity of quercetin and kaemferol against S. mutans biofilms, suggesting that quercetin and kaemferol might be considered as alternative anti-caries agents in searching novel anti-caries therapeutics.
目的
胡蜂巢（NV）是橄榄绿胡蜂、日本胡蜂和黄胡蜂的蜂巢。以前，我们已经表明，NV 的提取物和化学馏分在低于最低抑菌浓度（MIC）浓度下表现出显著抑制口腔细菌产酸的能力。在寻找 NV 中最有效的抗龋化合物时，我们进一步分离了 NV Chl/MeOH 馏分，得到了两种纯化化合物：槲皮素和山柰酚。本研究的目的是评估槲皮素和山柰酚对变形链球菌生物膜形成的有效性。
方法
测定 NV 衍生的槲皮素和山柰酚对变形链球菌的 MIC、最小生物膜抑制浓度（MBIC）和最小生物膜减少浓度（MBRC）。使用体外生物膜模型进一步检测抑制变形链球菌生物膜形成的效果。
结果
与阴性对照相比，槲皮素和山柰酚两种化合物均表现出抗生物膜活性。它们能够减少生物膜干重、总蛋白、通过集落形成单位（CFU）测量的活菌、不溶性和可溶性葡聚糖的形成。当用槲皮素和山柰酚处理生物膜时，原位培养 pH 值较低。与洗必泰相比，槲皮素和山柰酚对生物膜中变形链球菌的杀伤能力相当。
结论
本研究结果表明槲皮素和山柰酚对变形链球菌生物膜具有抑制活性，表明槲皮素和山柰酚可能被认为是寻找新型抗龋治疗方法的替代抗龋剂。
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