Department of Endocrinology, WHO Collaborating Center for the Diagnosis and Treatment of Thyroid Cancer and Other Thyroid Diseases, University of Pisa, Pisa, Italy.
J Endocrinol Invest. 2012 Sep;35(8):712-4. doi: 10.1007/BF03345804.
Thyroglobulin autoantibodies (TgAb) can develop in patients with subacute thyroiditis (SAT).
Comparison of the epitope pattern of TgAb of patients with SAT, Hashimoto's thyroiditis (HT) [autoimmune thyroid disease (AITD)] and non-toxic multinodular goiter (NTMG) (non-AITD).
Serum TgAb from 10 patients with SAT, 45 with HT, and 19 with NTMG were evaluated. Serum TgAb binding to Tg was inhibited by 4 recombinant human TgAb-Fab, recognizing Tg epitope regions A, B, C, and D. The ability of single TgAb-Fab to inhibit the binding of serum TgAb to Tg was evaluated in enzymelinked immunosorbent assay.
Levels of inhibition were different for all TgAb-Fab in the 3 groups of patients. Inhibition by region A TgAb-Fab in SAT [50.5 (30.3-62.5)%] (median and 25th to 75th percentiles) was similar to HT [49.0 (38.0-69.5)%] and significantly higher than in NTMG [25.0 (14.0-37.0)%]; by region B TgAb-Fab in SAT [0.0 (0.0-12.5)%] was significantly lower than in HT [28.0 (9.5-48.0)%] and similar to NTMG [9.0 (4.8-20.5)%]; by region C TgAb-Fab in SAT [9.5 (0.0-25.8)%] were similar to HT [23.0 (9.5-41)%] and NTMG [6.5 (1.7-21.5)%]; and by region D TgAb-Fab in SAT [0.0 (0.0-8.0)%] were lower than in HT [12.0 (1.0-28.5)%] and similar to NTMG [1.0 (0.0-5.0)%].
The epitope pattern of TgAb of SAT is restricted to the A region that is immunodominant in AITD and non-AITD. In the majority of patients with SAT, the autoimmune phenomena represent a non-specific and transient response to the release of thyroid antigens, rather than the expression of thyroid autoimmunity.
甲状腺球蛋白自身抗体（TgAb）可在亚急性甲状腺炎（SAT）患者中产生。
比较 SAT、桥本甲状腺炎（HT）[自身免疫性甲状腺疾病（AITD）]和非毒性多结节性甲状腺肿（NTMG）[非 AITD]患者 TgAb 的表位模式。
评估了 10 例 SAT、45 例 HT 和 19 例 NTMG 患者的血清 TgAb。通过 4 种识别 Tg 表位区域 A、B、C 和 D 的重组人 TgAb-Fab 抑制血清 TgAb 与 Tg 的结合。在酶联免疫吸附试验中评估单个 TgAb-Fab 抑制血清 TgAb 与 Tg 结合的能力。
所有 TgAb-Fab 在 3 组患者中的抑制水平不同。SAT 中区域 A TgAb-Fab 的抑制率[50.5（30.3-62.5）%]（中位数和 25%至 75%分位数）与 HT 相似[49.0（38.0-69.5）%]，明显高于 NTMG[25.0（14.0-37.0）%]；SAT 中区域 B TgAb-Fab 的抑制率[0.0（0.0-12.5）%]明显低于 HT[28.0（9.5-48.0）%]，与 NTMG 相似[9.0（4.8-20.5）%]；SAT 中区域 C TgAb-Fab 的抑制率[9.5（0.0-25.8）%]与 HT[23.0（9.5-41.0）%]和 NTMG[6.5（1.7-21.5）%]相似；SAT 中区域 D TgAb-Fab 的抑制率[0.0（0.0-8.0）%]低于 HT[12.0（1.0-28.5）%]，与 NTMG 相似[1.0（0.0-5.0）%]。
SAT 的 TgAb 表位模式局限于 A 区域，该区域在 AITD 和非 AITD 中是免疫优势的。在大多数 SAT 患者中，自身免疫现象代表甲状腺抗原释放的非特异性和短暂反应，而不是甲状腺自身免疫的表达。
