幽门螺杆菌感染固有免疫反应的体内和体外分析方法。

Methods for in vivo and in vitro analysis of innate immune responses to Helicobacter pylori infection.

作者信息

Patel Milan K, Ryan Glennice N, Cerny Anna M, Kurt-Jones Evelyn A

机构信息

Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.

出版信息

Methods Mol Biol. 2012;921:209-25. doi: 10.1007/978-1-62703-005-2_24.

DOI:10.1007/978-1-62703-005-2_24

PMID:23015507

Abstract

It is estimated that half of the world's population is infected by Helicobacter pylori (H. pylori) (Polk and Peek, Nat Rev Cancer 10:403-414, 2010; Peek et al., Physiol Rev 90:831-858, 2010). Following infection, H. pylori induces a chronic innate immune response that is thought to contribute to gastric complications. Due to the widespread prevalence of H. pylori, it is important to study the innate immune responses that result from the infection. A variety of in vitro and in vivo techniques have been developed by our laboratory to study this immune response (Fox et al., Am J Pathol 171:1520-1528, 2007; Kurt-Jones et al., Infect Immun 75:471-480, 2007; Kurt-Jones et al., J Endotoxin Res 10:419-424, 2004). These methods are described here.

摘要

据估计，全球一半的人口感染了幽门螺杆菌（H. pylori）（Polk和Peek，《自然评论癌症》10:403 - 414，2010年；Peek等人，《生理学评论》90:831 - 858，2010年）。感染后，幽门螺杆菌会引发一种慢性先天性免疫反应，这种反应被认为会导致胃部并发症。由于幽门螺杆菌的广泛流行，研究感染引发的先天性免疫反应很重要。我们实验室已经开发了多种体外和体内技术来研究这种免疫反应（Fox等人，《美国病理学杂志》171:1520 - 1528，2007年；Kurt - Jones等人，《感染与免疫》75:471 - 480，2007年；Kurt - Jones等人，《内毒素研究杂志》10:419 - 424，2004年）。这里将描述这些方法。