幽门螺杆菌尿素酶亚单位 B 诱导 Th17 细胞反应。

Induction of a Th17 cell response by Helicobacter pylori Urease subunit B.

机构信息

Department of Clinical Microbiology and Immunology, College of Medical Laboratory, The Third Military Medical University, Chongqing, PR China.

出版信息

Immunobiology. 2011 Jul;216(7):803-10. doi: 10.1016/j.imbio.2010.12.006. Epub 2010 Dec 25.

DOI:10.1016/j.imbio.2010.12.006

PMID:21269729

Abstract

Th17 cells represent a novel subset of CD4(+) T cells, which is associated with Helicobacter pylori infection. In the present study, we investigated the potential role of Urease subunit B (UreB) in the induction of Th17 cell response. Co-cultured splenic lymphocytes from H. pylori-infected mice with the recombinant UreB (rUreB) elevated IL-17 secretion and caused an increase in the number of Th17 cells. The expression of IL-6 and IL-23 p19 was significantly increased in rUreB-stimulated macrophages. Whole cell protein (WCP) of UreB-deficient strain (UreB(-) strain) induced less Th17 cell responses than that of wild-type strain. In addition, subcutaneous and intranasal immunization of rUreB elicited antigen-specific Th17 cell responses. Intranasal immunization of rUreB reduced H. pylori colonization in the stomach, which was closely related with the increased rUreB-specific Th17 cell responses. These results suggest that UreB is an important protein which is able to elicit Th17 cell responses against H. pylori both in vivo and in vitro.

摘要

Th17 细胞是一种新型的 CD4+T 细胞亚群，与幽门螺杆菌（Helicobacter pylori）感染有关。在本研究中，我们研究了尿素酶亚单位 B（UreB）在诱导 Th17 细胞反应中的潜在作用。与重组 UreB（rUreB）共培养来自 H. pylori 感染小鼠的脾淋巴细胞，可增加 IL-17 的分泌并增加 Th17 细胞的数量。rUreB 刺激的巨噬细胞中 IL-6 和 IL-23 p19 的表达显著增加。与野生型菌株相比，尿素酶缺陷型菌株（UreB(-) 菌株）的全细胞蛋白（WCP）诱导的 Th17 细胞反应较少。此外，rUreB 的皮下和鼻内免疫可引发针对 H. pylori 的抗原特异性 Th17 细胞反应。rUreB 的鼻内免疫可减少 H. pylori 在胃中的定植，这与 rUreB 特异性 Th17 细胞反应的增加密切相关。这些结果表明，UreB 是一种重要的蛋白，能够在体内和体外诱导针对 H. pylori 的 Th17 细胞反应。