• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

11-去氢表雄甾二酮内酯通过线粒体紊乱和内质网应激通路诱导人黑素瘤细胞凋亡。

Induction of apoptosis by 11-dehydrosinulariolide via mitochondrial dysregulation and ER stress pathways in human melanoma cells.

机构信息

Antai Medical Care Cooperation Antai Tian-Sheng Memorial Hospital, Pingtung 92842, Taiwan.

Department of Beauty Science, Meiho University, Pingtung 91202, Taiwan.

出版信息

Mar Drugs. 2012 Aug;10(8):1883-1898. doi: 10.3390/md10081883. Epub 2012 Aug 22.

DOI:10.3390/md10081883
PMID:23015779
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3447343/
Abstract

In this study the isolated compound 11-dehydrosinulariolide from soft coral Sinularia leptoclados possessed anti-proliferative, anti-migratory and apoptosis-inducing activities against A2058 melanoma cells. Anti-tumor effects of 11-dehydrosinulariolide were determined by MTT assay, cell migration assay and flow cytometry. Growth and migration of melanoma cells were dose-dependently inhibited by 2-8 μg/mL 11-dehydrosinulariolide. Flow cytometric data indicated that 11-dehydrosinulariolide induces both early and late apoptosis in melanoma cells. It was found that the apoptosis induced by 11-dehydrosinulariolide is relevant to mitochondrial-mediated apoptosis via caspase-dependent pathways, elucidated by loss of mitochondrial membrane potential (∆Ψm), release of cytochrome C, activation of caspase-3/-9 and Bax as well as suppression of Bcl-2/Bcl-xL. The cleavage of PARP-1 suggested partial involvement of caspase-independent pathways. Immunoblotting data displayed up-regulations of PERK/eIF2α/ATF4/CHOP and ATF6/CHOP coupling with elevation of ER stress chaperones GRP78, GRP94, calnexin, calreticulin and PDI, implicating the involvement of these factors in ER stress-mediated apoptosis induced by 11-dehydrosinulariolide. The abolishment of apoptotic events after pre-treatment with salubrinal indicated that ER stress-mediated apoptosis is also induced by 11-dehydrosinulariolide against melanoma cells. The data in this study suggest that 11-dehydrosinulariolide potentially induces apoptosis against melanoma cells via mitochondrial dysregulation and ER stress pathways.

摘要

在这项研究中，从软珊瑚 Sinularia leptoclados 中分离得到的单体化合物 11-脱水辛可尼醇内酯对 A2058 黑色素瘤细胞具有抗增殖、抗迁移和诱导凋亡的活性。通过 MTT 测定、细胞迁移测定和流式细胞术测定了 11-脱水辛可尼醇内酯的抗肿瘤作用。2-8μg/ml 的 11-脱水辛可尼醇内酯可剂量依赖性地抑制黑色素瘤细胞的生长和迁移。流式细胞术数据表明，11-脱水辛可尼醇内酯诱导黑色素瘤细胞发生早期和晚期凋亡。研究发现，11-脱水辛可尼醇内酯诱导的凋亡与通过 caspase 依赖性途径的线粒体介导的凋亡有关，这是通过线粒体膜电位（∆Ψm）丧失、细胞色素 C 释放、caspase-3/-9 和 Bax 的激活以及 Bcl-2/Bcl-xL 的抑制来阐明的。PARP-1 的裂解表明部分涉及 caspase 非依赖性途径。免疫印迹数据显示 PERK/eIF2α/ATF4/CHOP 和 ATF6/CHOP 的上调与内质网应激伴侣 GRP78、GRP94、钙连蛋白、钙网蛋白和 PDIA 的升高相关，表明这些因素参与了 11-脱水辛可尼醇内酯诱导的内质网应激介导的凋亡。用 salubrinal 预处理后凋亡事件的消除表明，内质网应激介导的凋亡也是 11-脱水辛可尼醇内酯对黑色素瘤细胞的诱导作用。本研究数据表明，11-脱水辛可尼醇内酯可能通过线粒体失调和内质网应激途径诱导黑色素瘤细胞凋亡。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5ef/3447343/75008978b287/marinedrugs-10-01883-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5ef/3447343/92fa1f19dbae/marinedrugs-10-01883-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5ef/3447343/c8008c53b054/marinedrugs-10-01883-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5ef/3447343/9a076284af02/marinedrugs-10-01883-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5ef/3447343/5a56afeb1b6a/marinedrugs-10-01883-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5ef/3447343/75008978b287/marinedrugs-10-01883-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5ef/3447343/92fa1f19dbae/marinedrugs-10-01883-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5ef/3447343/c8008c53b054/marinedrugs-10-01883-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5ef/3447343/9a076284af02/marinedrugs-10-01883-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5ef/3447343/5a56afeb1b6a/marinedrugs-10-01883-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5ef/3447343/75008978b287/marinedrugs-10-01883-g005.jpg

相似文献

1
Induction of apoptosis by 11-dehydrosinulariolide via mitochondrial dysregulation and ER stress pathways in human melanoma cells.11-去氢表雄甾二酮内酯通过线粒体紊乱和内质网应激通路诱导人黑素瘤细胞凋亡。
Mar Drugs. 2012 Aug;10(8):1883-1898. doi: 10.3390/md10081883. Epub 2012 Aug 22.
2
Induction of apoptosis by sinulariolide from soft coral through mitochondrial-related and p38MAPK pathways on human bladder carcinoma cells.辛拉瑞醇内酯通过线粒体相关途径和 p38MAPK 通路诱导人膀胱癌细胞凋亡。
Mar Drugs. 2012 Dec 18;10(12):2893-911. doi: 10.3390/md10122893.
3
Sinulariolide induced hepatocellular carcinoma apoptosis through activation of mitochondrial-related apoptotic and PERK/eIF2α/ATF4/CHOP pathway.辛纳利醇内酯通过激活线粒体相关凋亡途径和 PERK/eIF2α/ATF4/CHOP 通路诱导肝癌细胞凋亡。
Molecules. 2013 Aug 22;18(9):10146-61. doi: 10.3390/molecules180910146.
4
A Soft Coral-Derived Compound, 11-Dehydrosinulariolide, Induces G2/M Cell Cycle Arrest and Apoptosis in Small Cell Lung Cancer.一种源自软珊瑚的化合物 11-脱水辛可尼醇内酯诱导小细胞肺癌细胞发生 G2/M 期细胞周期阻滞和凋亡。
Mar Drugs. 2018 Nov 30;16(12):479. doi: 10.3390/md16120479.
5
Flaccidoxide-13-Acetate-Induced Apoptosis in Human Bladder Cancer Cells is through Activation of p38/JNK, Mitochondrial Dysfunction, and Endoplasmic Reticulum Stress Regulated Pathway.Flaccidoxide-13-Acetate 诱导人膀胱癌细胞凋亡是通过激活 p38/JNK、线粒体功能障碍和内质网应激调控途径实现的。
Mar Drugs. 2019 May 13;17(5):287. doi: 10.3390/md17050287.
6
The Effect of Bornyl -4-Hydroxycinnamate on Melanoma Cell Apoptosis Is Associated with Mitochondrial Dysfunction and Endoplasmic Reticulum Stress.莰烯-4-羟基肉桂酸酯对黑素瘤细胞凋亡的影响与线粒体功能障碍和内质网应激有关。
Int J Mol Sci. 2018 May 4;19(5):1370. doi: 10.3390/ijms19051370.
7
Proteomic investigation of the sinulariolide-treated melanoma cells A375: effects on the cell apoptosis through mitochondrial-related pathway and activation of caspase cascade.对辛拉瑞醇处理的黑色素瘤细胞 A375 的蛋白质组学研究：通过线粒体相关途径对细胞凋亡的影响和半胱天冬酶级联的激活。
Mar Drugs. 2013 Jul 22;11(7):2625-42. doi: 10.3390/md11072625.
8
Proteomic profiling of the 11-dehydrosinulariolide-treated oral carcinoma cells Ca9-22: effects on the cell apoptosis through mitochondrial-related and ER stress pathway.11-去氢表小檗碱处理口腔癌细胞 Ca9-22 的蛋白质组学分析：通过线粒体相关和内质网应激途径对细胞凋亡的影响。
J Proteomics. 2012 Oct 22;75(18):5578-89. doi: 10.1016/j.jprot.2012.07.037. Epub 2012 Aug 3.
9
Proteomic investigation of anti-tumor activities exerted by sinularin against A2058 melanoma cells.硅珊瑚素抗 A2058 黑素瘤细胞活性的蛋白质组学研究。
Electrophoresis. 2012 Apr;33(7):1139-52. doi: 10.1002/elps.201100462.
10
16-O-caffeoyl-16-hydroxylhexadecanoic acid, a medicinal plant-derived phenylpropanoid, induces apoptosis in human hepatocarcinoma cells through ROS-dependent endoplasmic reticulum stress.16-O-咖啡酰基-16-羟基十六烷酸，一种药用植物衍生的苯丙素，通过 ROS 依赖性内质网应激诱导人肝癌细胞凋亡。
Phytomedicine. 2018 Mar 1;41:33-44. doi: 10.1016/j.phymed.2018.01.024. Epub 2018 Jan 31.

引用本文的文献

1
Natural products as therapeutics for malignant melanoma: preclinical evidence and mechanism.天然产物作为恶性黑色素瘤的治疗药物：临床前证据与机制
Front Pharmacol. 2025 Aug 26;16:1641838. doi: 10.3389/fphar.2025.1641838. eCollection 2025.
2
Selective apoptosis induction by antimicrobial peptide TP4 in MCF-7 breast cancer cells.抗菌肽TP4在MCF-7乳腺癌细胞中诱导选择性细胞凋亡
Sci Rep. 2025 Apr 29;15(1):15061. doi: 10.1038/s41598-025-97906-3.
3
Nutritional Health Connection of Algae and its Pharmaceutical Value as Anticancer and Antioxidant Constituents of Drugs.

本文引用的文献

1
Antiproliferative effects of goniothalamin on Ca9-22 oral cancer cells through apoptosis, DNA damage and ROS induction.岗柃亭通过诱导细胞凋亡、DNA 损伤和 ROS 产生对 Ca9-22 口腔癌细胞的抗增殖作用。
Mutat Res. 2012 Sep 18;747(2):253-8. doi: 10.1016/j.mrgentox.2012.06.003. Epub 2012 Jun 18.
2
Adjuvant therapy for melanoma.黑色素瘤的辅助治疗。
Cancer J. 2012 Mar-Apr;18(2):192-202. doi: 10.1097/PPO.0b013e31824f118b.
3
An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells.
藻类的营养健康关联及其作为药物抗癌和抗氧化成分的药用价值。
Recent Pat Biotechnol. 2025;19(1):19-34. doi: 10.2174/0118722083287672240321081428.
4
Soft Corals-derived Dihydrosinularin Attenuates Neuronal Apoptosis in 6-hydroxydopamine-induced Cell Model of Parkinson's Disease by Regulating the PI3K Pathway.软珊瑚来源的二氢柳珊瑚素通过调节PI3K信号通路减轻6-羟基多巴胺诱导的帕金森病细胞模型中的神经元凋亡。
Curr Med Chem. 2024 Sep 3;32(8):1606-20. doi: 10.2174/0109298673323198240823070219.
5
From Beach to the Bedside: Harnessing Mitochondrial Function in Human Diseases Using New Marine-Derived Strategies.从海滩到床边：利用新的海洋衍生策略在人类疾病中利用线粒体功能。
Int J Mol Sci. 2024 Jan 9;25(2):834. doi: 10.3390/ijms25020834.
6
Deep-Sea Sponges and Corals off the Western Coast of Florida-Intracellular Mechanisms of Action of Bioactive Compounds and Technological Advances Supporting the Drug Discovery Pipeline.佛罗里达西海岸深海海绵和珊瑚——生物活性化合物的细胞内作用机制和支持药物发现管道的技术进步。
Mar Drugs. 2023 Nov 28;21(12):615. doi: 10.3390/md21120615.
7
Cudraflavone B induces human glioblastoma cells apoptosis via ER stress-induced autophagy.瓜馥木黄酮 B 通过内质网应激诱导的自噬诱导人胶质母细胞瘤细胞凋亡。
BMC Neurosci. 2023 Jan 31;24(1):10. doi: 10.1186/s12868-023-00778-4.
8
Towards Sustainable Medicinal Resources through Marine Soft Coral Aquaculture: Insights into the Chemical Diversity and the Biological Potential.通过海洋软珊瑚养殖实现可持续药用资源：对化学多样性和生物潜力的洞察。
Mar Drugs. 2022 Oct 14;20(10):640. doi: 10.3390/md20100640.
9
Corn Silk () Induced Apoptosis in Human Breast Cancer (MCF-7) Cells via the ROS-Mediated Mitochondrial Pathway.玉米须通过 ROS 介导的线粒体途径诱导人乳腺癌（MCF-7）细胞凋亡。
Oxid Med Cell Longev. 2019 Oct 20;2019:9789241. doi: 10.1155/2019/9789241. eCollection 2019.
10
Effects of paclitaxel and cisplatin on ovarian follicle development.紫杉醇和顺铂对卵巢卵泡发育的影响。
Arch Med Sci. 2019 Oct;15(6):1510-1519. doi: 10.5114/aoms.2019.81730. Epub 2019 Jan 30.
研究软珊瑚 Sarcophyton crassocaule 中的 13-乙酰基鲨鱼软骨素对膀胱癌细胞的细胞毒性作用。
Mar Drugs. 2011 Dec;9(12):2622-2642. doi: 10.3390/md9122622. Epub 2011 Dec 13.
4
Advances in the treatment of ovarian cancer: a potential role of antiinflammatory phytochemicals.卵巢癌治疗进展：抗炎植物化学物质的潜在作用
Discov Med. 2012 Jan;13(68):7-17.
5
Antitumor promoting potential of selected phytochemicals derived from spices: a review.从香料中提取的部分植物化学成分的抗肿瘤促进潜力：综述。
Eur J Cancer Prev. 2012 Mar;21(2):205-15. doi: 10.1097/CEJ.0b013e32834a7f0c.
6
Proteomic analysis of anti-tumor effects of 11-dehydrosinulariolide on CAL-27 cells.对 11-脱水金黄裸壳菌素抗 CAL-27 细胞肿瘤作用的蛋白质组学分析。
Mar Drugs. 2011;9(7):1254-1272. doi: 10.3390/md9071254. Epub 2011 Jul 14.
7
A lipid-modified estrogen derivative that treats breast cancer independent of estrogen receptor expression through simultaneous induction of autophagy and apoptosis.一种脂修饰的雌激素衍生物，通过同时诱导自噬和细胞凋亡来治疗乳腺癌，而不依赖于雌激素受体表达。
Mol Cancer Res. 2011 Mar;9(3):364-74. doi: 10.1158/1541-7786.MCR-10-0526. Epub 2011 Feb 2.
8
Melanoma staging and sentinel lymph node biopsy.黑色素瘤分期与前哨淋巴结活检
Acta Dermatovenerol Croat. 2010;18(4):279-88.
9
Chemotherapy resistance abrogation in metastatic melanoma.转移性黑色素瘤中化疗耐药性的消除
Clin Adv Hematol Oncol. 2010 Apr;8(4):259-66.
10
p38 MAPK and NF-kappaB pathways are involved in naphtho[1,2-b] furan-4,5-dione induced anti-proliferation and apoptosis of human hepatoma cells.p38MAPK 和 NF-κB 通路参与萘[1,2-b]呋喃-4,5-二酮诱导的人肝癌细胞增殖抑制和凋亡。
Cancer Lett. 2010 Sep 1;295(1):92-9. doi: 10.1016/j.canlet.2010.02.017. Epub 2010 Mar 29.