Antai Medical Care Cooperation Antai Tian-Sheng Memorial Hospital, Pingtung 92842, Taiwan.
National Museum of Marine Biology and Aquarium, Pingtung 94446, Taiwan.
Mar Drugs. 2011 Dec;9(12):2622-2642. doi: 10.3390/md9122622. Epub 2011 Dec 13.
Active compounds from natural products have been widely studied. The anti-tumor effects of 13-acetoxysarcocrassolide isolated from Formosan soft coral Sarcophyton crassocaule on bladder cancer cells were examined in this study. An MTT assay showed that 13-acetoxysarcocrassolide was cytotoxic to bladder female transitional cancer (BFTC) cells. We determined that the BFTC cells underwent cell death through apoptosis by flow cytometry. Due to the highly-migratory nature of the BFTC cells, the ability of 13-acetoxysarcocrassolide to stop their migration was assessed by a wound healing assay. To determine which proteins were affected in the BFTC cells upon treatment, a comparative proteomic analysis was performed. By LC-MS/MS analysis, we identified that 19 proteins were up-regulated and eight were down-regulated. Seven of the proteins were confirmed by western blotting analysis. This study reveals clues to the potential mechanism of the cytotoxic effects of 13-acetoxysarcocrassolide on BFTC cells. Moreover, it suggests that PPT1 and hnRNP F could be new biomarkers for bladder cancer. The results of this study are also helpful for the diagnosis, progression monitoring and therapeutic strategies of transitional cell tumors.
从天然产物中提取的活性化合物已得到广泛研究。本研究考察了从台湾软珊瑚 Sarcophyton crassocaule 中分离得到的 13-乙酰基 sarcocrassolide 对膀胱癌细胞的抗肿瘤作用。MTT 检测结果表明，13-乙酰基 sarcocrassolide 对膀胱女性移行癌细胞（BFTC）具有细胞毒性。我们通过流式细胞术确定 BFTC 细胞通过细胞凋亡发生死亡。由于 BFTC 细胞具有高度迁移的特性，通过划痕愈合试验评估 13-乙酰基 sarcocrassolide 阻止其迁移的能力。为了确定 BFTC 细胞在治疗后哪些蛋白质受到影响，进行了比较蛋白质组学分析。通过 LC-MS/MS 分析，我们鉴定出 19 种蛋白上调，8 种蛋白下调。通过 Western blot 分析验证了其中 7 种蛋白。本研究揭示了 13-乙酰基 sarcocrassolide 对 BFTC 细胞细胞毒性作用的潜在机制的线索。此外，研究结果还提示 PPT1 和 hnRNP F 可能成为膀胱癌的新生物标志物。本研究结果有助于膀胱癌的诊断、进展监测和治疗策略。
