Lane R S
University of California, Berkeley.
Am J Trop Med Hyg. 1990 Jan;42(1):75-82. doi: 10.4269/ajtmh.1990.42.75.
Attempts to infect juvenile and adult western fence lizards (Sceloporus occidentalis) with the Lyme borreliosis spirochete (Borrelia burgdorferi) were largely unsuccessful. Spirochetes could not be isolated from the blood and various tissues of 14 lizards 21-32 days after they had been inoculated ip (n = 8) or sc (n = 6) with 10(6) or 10(8) B. burgdorferi representing 3 tick isolates, although 1 lizard apparently developed a transitory spirochetemia lasting 2 days. Similarly, spirochetes could not be detected in the blood or tissues of 5 lizards fed upon by 2- greater than 8 infected larvae or nymphs of the western black-legged tick (Ixodes pacificus). Sixty-five blood samples from 59 lizards in an endemic area and various tissues from 20 of the same lizards were also assayed for B. burgdorferi with negative results. The implications of these findings for the maintenance of this spirochete in natural foci are discussed.
尝试用莱姆病螺旋体（伯氏疏螺旋体）感染幼年和成年西部围栏蜥蜴（西部强棱蜥），结果大多不成功。在用代表3种蜱虫分离株的10⁶或10⁸个伯氏疏螺旋体经腹腔注射（n = 8）或皮下注射（n = 6）接种14只蜥蜴21 - 32天后，无法从其血液和各种组织中分离出螺旋体，尽管有1只蜥蜴显然出现了持续2天的短暂螺旋体血症。同样，在被2只及以上超过8只感染的西部黑腿蜱（太平洋硬蜱）幼虫或若虫叮咬的5只蜥蜴的血液或组织中，也未检测到螺旋体。还对来自流行地区的59只蜥蜴的65份血液样本以及其中20只相同蜥蜴的各种组织进行了伯氏疏螺旋体检测，结果均为阴性。讨论了这些发现对该螺旋体在自然疫源地维持的意义。
