Huang Yu-Bin, Zou Su-Qi, Yin Wu, Wang Kun, Wang Han, Hu Bing
CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
Yi Chuan. 2012 Sep;34(9):1193-201.
As a means of visual function testing and visual related mutants screening, the optokinetic response (OKR) and the optomotor response (OMR) behaviour tests are simple and effective tools for visual functional testing, which have been widely used in studying zebrafish larvae. However, adult zebrafish OKR analysis method is rarely reported. In this study, the methods of inducing adult zebrafish OKR behaviour, as well as tracking the movement of eyes using Pattern Match approaches, are presented. The quantitative measurement of the adult zebrafish OKR behaviour was successfully established. Using these methods, the binocular vision area was found to make a certain contribution to OKR behaviour. Moreover, the monocular vision of adult zebrafish showed a certain degree of directional sensitivity to moving gratings. Such approaches can also be applied to the zebrafish larvae OKR. The abnormal OKR behaviour phenomenon of period1b mutant larvae fish was detected.
作为视觉功能测试和视觉相关突变体筛选的一种手段,视动反应(OKR)和视动运动反应(OMR)行为测试是视觉功能测试的简单有效工具,已广泛应用于斑马鱼幼体研究。然而,成年斑马鱼OKR分析方法鲜有报道。在本研究中,介绍了诱导成年斑马鱼OKR行为以及使用模式匹配方法跟踪眼睛运动的方法。成功建立了成年斑马鱼OKR行为的定量测量方法。使用这些方法,发现双眼视觉区域对OKR行为有一定贡献。此外,成年斑马鱼的单眼视觉对移动光栅表现出一定程度的方向敏感性。这些方法也可应用于斑马鱼幼体的OKR。检测到period1b突变体幼鱼的OKR行为异常现象。