University of Zurich, Institute of Zoology, Neuroscience Center Zurich and Center for Integrative Human Physiology, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
J Neurosci Methods. 2010 Jan 30;186(1):29-34. doi: 10.1016/j.jneumeth.2009.10.020. Epub 2009 Nov 10.
Small teleost fish are increasingly used for studying the genetic basis of vision. In particular, zebrafish (Danio rerio) and medaka (Oryzias latipes) are commonly used vertebrate model organisms in developmental research, including research on the development of visual function. A multitude of behavior-based visual tests are established for larvae that have been successfully used to identify and characterize visual defects in genetically manipulated strains of these species. Testing the visual system of adult fish has proven to be more difficult for a number of reasons, including complications in restraining fish, or shoaling and dominance behavior interfering with visual behavior in population screening assays. In this paper, we present a simple and cost-effective method to quantitatively measure the optokinetic response (OKR) of individual adult zebrafish and medaka, which can be used to characterize visual capabilities of adult fish. This method can be applied to any fish species of similar size.
小型硬骨鱼越来越多地被用于研究视觉的遗传基础。特别是斑马鱼（Danio rerio）和青鳉（Oryzias latipes），作为脊椎动物模式生物，常用于发育研究，包括视觉功能发育的研究。已经建立了许多基于行为的幼鱼视觉测试方法，这些方法已成功用于鉴定和描述这些物种的遗传操作品系中的视觉缺陷。由于多种原因，成年鱼类的视觉系统测试变得更加困难，包括限制鱼类的复杂性，或群体筛选试验中鱼的集群和优势行为干扰视觉行为。在本文中，我们提出了一种简单且具有成本效益的方法，可定量测量成年斑马鱼和青鳉的光控反应（OKR），可用于描述成年鱼类的视觉能力。这种方法可应用于任何大小相似的鱼类物种。
