利用视动反应研究斑马鱼的视觉功能。

Using the optokinetic response to study visual function of zebrafish.

作者信息

Zou Su-Qi, Yin Wu, Zhang Ming-Jing, Hu Chun-Rui, Huang Yu-Bin, Hu Bing

机构信息

Laboratory of Neurodevelopment and Repair, Department of Neurobiology and Biophysics, School of Life, University of Science and Technology of China, China.

出版信息

J Vis Exp. 2010 Feb 2(36):1742. doi: 10.3791/1742.

DOI:10.3791/1742

PMID:20125082

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2818711/

Abstract

Optokinetic response (OKR) is a behavior that an animal vibrates its eyes to follow a rotating grating around it. It has been widely used to assess the visual functions of larval zebrafish. Nevertheless, the standard protocol for larval fish is not yet readily applicable in adult zebrafish. Here, we introduce how to measure the OKR of adult zebrafish with our simple custom-built apparatus using a new protocol which is established in our lab. Both our apparatus and step-by-step procedure of OKR in adult zebrafish are illustrated in this video. In addition, the measurements of the larval OKR, as well as the optomotor response (OMR) test of adult zebrafish, are also demonstrated in this video. This OKR assay of adult zebrafish in our experiment may last for up to 4 hours. Such OKR test applied in adult fish will benefit to visual function investigation more efficiently when the adult fish vision system is manipulated.

摘要

视动反应（OKR）是一种动物振动眼睛以跟随其周围旋转光栅的行为。它已被广泛用于评估斑马鱼幼体的视觉功能。然而，幼体鱼类的标准方案尚未 readily 适用于成年斑马鱼。在此，我们介绍如何使用我们实验室建立的新方案，通过我们简单的定制设备来测量成年斑马鱼的 OKR。本视频展示了我们用于成年斑马鱼 OKR 的设备和逐步操作过程。此外，本视频还展示了幼体 OKR 的测量以及成年斑马鱼的视动反应（OMR）测试。我们实验中成年斑马鱼的这种 OKR 测定可能持续长达 4 小时。当成年鱼视觉系统受到操纵时，应用于成年鱼的这种 OKR 测试将更有效地有助于视觉功能研究。