鉴定巴西固氮螺菌 Sp7 的极性鞭毛鞭毛蛋白的 O-连接重复聚糖链。
Identification of an O-linked repetitive glycan chain of the polar flagellum flagellin of Azospirillum brasilense Sp7.
机构信息
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia.
出版信息
Carbohydr Res. 2012 Nov 1;361:127-32. doi: 10.1016/j.carres.2012.08.019. Epub 2012 Sep 5.
This is the first report to have identified an O-linked repetitive glycan in bacterial flagellin, a structural protein of the flagellum. Studies by sugar analysis, Smith degradation, (1)H and (13)C NMR spectroscopy, and mass spectrometry showed that the glycan chains of the polar flagellum flagellin of the plant-growth-promoting rhizobacterium Azospirillum brasilense Sp7 are represented by a polysaccharide with a molecular mass of 7.7 kDa, which has a branched tetrasaccharide repeating unit of the following structure:
这是首次在细菌鞭毛蛋白（鞭毛的结构蛋白）中鉴定出 O-连接重复聚糖。通过糖分析、Smith 降解、（1）H 和（13）C NMR 光谱以及质谱研究表明，植物促生根瘤菌 Azospirillum brasilense Sp7 的极性鞭毛鞭毛蛋白的聚糖链由一种分子量为 7.7 kDa 的多糖组成，其具有以下结构的支化四糖重复单元：
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