巴西固氮螺菌Sp7中编码侧生鞭毛鞭毛蛋白的结构基因laf1的表达。
Expression of the structural gene, laf1, encoding the flagellin of the lateral flagella in Azospirillum brasilense Sp7.
作者信息
Moens S, Schloter M, Vanderleyden J
机构信息
F.A. Janssens Laboratory of Genetics, Katholieke Universiteit Leuven, Heverlee, Belgium.
出版信息
J Bacteriol. 1996 Aug;178(16):5017-9. doi: 10.1128/jb.178.16.5017-5019.1996.
Abstract
The induction of the lateral flagella of Azospirillum brasilense Sp7 was studied by using a translational fusion between the laf1 promoter and gusA. The fusion was induced when cells were grown on solid media but not when they were grown in broth. The fusion was also induced by incubation of liquid-grown cells with an anti-polar flagellum polyclonal antiserum. Hindrance of polar-flagellum rotation is suggested to be the signal for this induction.
摘要
利用laf1启动子与gusA之间的翻译融合，研究了巴西固氮螺菌Sp7侧生鞭毛的诱导情况。当细胞在固体培养基上生长时，该融合被诱导，而在肉汤中生长时则不被诱导。用抗极生鞭毛多克隆抗血清孵育液体培养的细胞也可诱导该融合。极生鞭毛旋转受阻被认为是这种诱导的信号。
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