Rothuizen J, van den Brom W E
Department of Clinical Sciences of Companion Animals, University of Utrecht, The Netherlands.
Am J Vet Res. 1990 Feb;51(2):253-6.
In 25 dogs with spontaneous cholestatic disease, the hepatobiliary dynamics were evaluated by use of scintigraphy and a 99mTc-labeled iminodiacetate (IDA) derivative. Hyperbilirubinemia existed in all dogs, with serum total bilirubin concentration ranging from 6 to 262 mumol/L. An appropriate compartmental model was used to characterize the liver time-activity curves. Model-dependent variables for hepatic uptake and biliary excretion of radiolabeled IDA were found to reliably represent the underlying physiologic processes. Measurements directly derived from the liver time-activity curves of IDA, representing the moments of accumulation of 50 and 95% of the maximal hepatic activity did not accurately represent the hepatic uptake by being significantly influenced by biliary excretion and by competition of renal excretion. The time-interval between 95% and 50% of the maximal activity in the excretory phase proved to be a quantitative characteristic of bile flow in all instances. Compartmental analysis of 99mTc-IDA excretory scintigraphy characterized bile flow quantitatively in clinically normal dogs and in dogs with cholestasis. The method permitted the clinical evaluation of cholestasis based on quantitative, instead of the usual qualitative, and on functional, instead of phenomenologic, criteria.
在25只患有自发性胆汁淤积性疾病的犬中，采用闪烁扫描法和一种99mTc标记的亚氨基二乙酸（IDA）衍生物对肝胆动力学进行评估。所有犬均存在高胆红素血症，血清总胆红素浓度范围为6至262μmol/L。使用合适的房室模型来描述肝脏时间-活性曲线。发现放射性标记的IDA在肝脏摄取和胆汁排泄方面的模型相关变量能够可靠地反映潜在的生理过程。直接从IDA的肝脏时间-活性曲线得出的测量值，即代表最大肝脏活性50%和95%积累时刻的值，由于受到胆汁排泄和肾排泄竞争的显著影响，不能准确反映肝脏摄取情况。排泄期最大活性的95%和50%之间的时间间隔在所有情况下都被证明是胆汁流量的一个定量特征。99mTc-IDA排泄性闪烁扫描的房室分析在临床正常犬和胆汁淤积犬中对胆汁流量进行了定量表征。该方法允许基于定量而非通常的定性标准，以及基于功能而非现象学标准对胆汁淤积进行临床评估。
