Department of Analytical Chemistry, Faculty of Chemistry, University of Alcalá, Ctra. Madrid-Barcelona, Km. 33.600, E-28871 Alcalá de Henares, Madrid, Spain.
J Proteomics. 2012 Apr 18;75(8):2396-403. doi: 10.1016/j.jprot.2012.02.020. Epub 2012 Feb 23.
Different types of extraction protocols are described for identifying proteins in seed and pulp of olive (Olea europea), by employing both conventional extraction methods and capture with ProteoMiner as well as with in house-made combinatorial peptide ligand libraries (HM-CPLLs) at pH 7.4 and at pH 2.2. Thanks to the use of CPLLs, able to dramatically amplify the signal of low-abundance species, a quite large number of compounds has been indeed identified: 61 in the seed (vs. only four reported in current literature) and 231 in the pulp (vs. 56 described so far), the deepest investigation up to the present of the olive proteome. In the seed, it highlights the presence of seed storage proteins, oleosins and histones. In the pulp, the allergenic thaumatin-like protein (Ole e 13) was confirmed, among the other 231, as the most abundant protein in the olive pulp. The present research has also been undertaken with the aim of identifying proteins in olive oil and ascertaining the relative contribution of seed and pulp proteins in their presence, if any, in oils.
描述了不同类型的提取方案，用于通过使用常规提取方法和使用 ProteoMiner 以及在 pH 7.4 和 pH 2.2 下使用内部组合肽配体文库 (HM-CPLL) 从橄榄 (Olea europaea) 的种子和果肉中鉴定蛋白质。由于使用了能够显著放大低丰度物质信号的 CPLL，实际上已经鉴定出相当多的化合物：种子中 61 种（而目前文献中仅报道了 4 种），果肉中 231 种（迄今为止已描述了 56 种），这是迄今为止对橄榄蛋白质组的最深入研究。在种子中，它突出了种子贮藏蛋白、油体蛋白和组蛋白的存在。在果肉中，确认了过敏原辣根样蛋白（Ole e 13）是橄榄果肉中最丰富的蛋白之一，除其他 231 种蛋白之外。本研究还旨在鉴定橄榄油中的蛋白质，并确定种子和果肉蛋白质在其存在时的相对贡献（如果有的话）。
