J Mass Spectrom. 2012 Oct;47(10):1347-52. doi: 10.1002/jms.3086.
Our goal was to compare two popular analytical techniques used nowadays in proteomic investigations for proteins/peptides sequencing and identification, a widely used nanoLC-MS/MS approach applied in the bottom-up proteomics and electron transfer dissociation/proton transfer reaction fragmentation preferably used when top-down strategy is applied. Comparison was carried out with the aid of the ESI-quadrupole ion-trap instrument using the following criteria: total time of analysis including sample preparation, sequence coverage, Mascot scoring, capability to detect modifications, quality of the results as a function of protein molecular weight and sample consumption.
我们的目标是比较两种现今在蛋白质组学研究中用于蛋白质/肽测序和鉴定的常用分析技术，一种是广泛应用于 Bottom-up 蛋白质组学的 nanoLC-MS/MS 方法，另一种是在应用 Top-down 策略时首选的电子转移解离/质子转移反应碎裂。使用 ESI-四极离子阱仪器，通过以下标准进行比较：包括样品制备在内的总分析时间、序列覆盖率、Mascot 评分、检测修饰的能力、结果质量与蛋白质分子量和样品消耗的关系。
