Chen Daxia, Zhang Xue, Li Longyun
Chongqing Engineering Research Center of Lonicea Flos, Chongqing Research Center of Techniques and Engineering for Chinese Materia Medica Fine Variety Breeding and Evaluation, Institute of Material Medical Planting, Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China.
Zhongguo Zhong Yao Za Zhi. 2012 Jul;37(13):1912-6.
To study genetic diversity and genetic relationships among Lonicera macranthoides cultivars.
Five cultivars were estimated by ISSR and SRAP. The data of amplified bands were analyzed by Treeconw software. The system diagram of genetic relationship was built by UPGMA.
Twenty ISSR primers amplified 186 bands with 103 (54.63%) polymorphic bands and 58 SRAP primer combinations amplified 591 bands with 347(55.46%) polymorphic bands. Genetic distance ranges were 0.058 4-0.230 8 (by ISSRs) and 0.1071-0.2611 (by SRAPs). Both ISSR and SRAP analyses revealed a middle level of genetic diversity in L. macranthoides cultivars. The dendrograms based on SRAP and ISSR markers were not all the same.
The genetic diversity of L. macranthoides cultivars is middle. ISSR and SRAP markers can be effectively applied to genetic analysis in L. macranthoides cultivars.
研究灰毡毛忍冬品种间的遗传多样性及亲缘关系。
采用ISSR和SRAP标记对5个灰毡毛忍冬品种进行分析。利用Treeconw软件对扩增条带数据进行分析，采用UPGMA构建亲缘关系系统图。
20条ISSR引物共扩增出186条带，其中多态性条带103条（54.63%）；58对SRAP引物组合共扩增出591条带，其中多态性条带347条（55.46%）。ISSR标记的遗传距离范围为0.058 4 - 0.230 8，SRAP标记的遗传距离范围为0.1071 - 0.2611。ISSR和SRAP分析均显示灰毡毛忍冬品种具有中等水平的遗传多样性。基于SRAP和ISSR标记构建的聚类图不完全相同。
灰毡毛忍冬品种的遗传多样性处于中等水平。ISSR和SRAP标记可有效应用于灰毡毛忍冬品种的遗传分析。
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