Liu Ying, Liu Chun-Sheng
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, China.
Zhong Yao Cai. 2012 Apr;35(4):528-31.
To reveal the temporal and spatial specificity of the expression of beta-AS gene of Glycyrriza uralensis.
Used PCR to obtain the cDNA of beta-AS gene of Glycyrriza uralensis at different time and from different part and Gel-Pro to carry out the densitometric analysis of the electrophoretic band, then calculate the relative expression.
The spatial specificity experiment showed that beta-AS gene didn't express in the overground part of Glycyrriza uralensis,while in the underground part,the expression of beta-AS in root tip was higher than that of rootstock. And the temporal specificity experiment showed that the expression of beta-AS gene of Glycyrriza uralensis could be divided into 4 stages. From December to February, the expression of beta-AS gene was under the detection limit. From March to May, beta-AS gene began to express. From May to September,the expression of beta-AS gene kept at a high level. And in October and November the expression of beta-AS gene began to decrease.
When the beta-AS gene of Glycyrriza uralensis is researched, the root tip is the suitable plant material and May, June, August and September are the right acquisition time.
揭示乌拉尔甘草β-AS基因表达的时空特异性。
采用PCR技术获取乌拉尔甘草不同时期、不同部位的β-AS基因cDNA，并用Gel-Pro软件对电泳条带进行光密度分析，进而计算相对表达量。
空间特异性实验表明，β-AS基因在乌拉尔甘草地上部分不表达，而在地下部分，根尖中β-AS的表达高于根茎。时间特异性实验表明，乌拉尔甘草β-AS基因的表达可分为4个阶段。12月至2月，β-AS基因的表达低于检测限。3月至5月，β-AS基因开始表达。5月至9月，β-AS基因的表达维持在较高水平。10月和11月，β-AS基因的表达开始下降。
研究乌拉尔甘草β-AS基因时，根尖是合适的植物材料，5月、6月、8月和9月是适宜的取材时间。
