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注意:一种基于通用现场可编程门阵列开发板的4纳秒硬件光子相关器,采用紧凑设置实现用于荧光相关光谱分析。

Note: a 4 ns hardware photon correlator based on a general-purpose field-programmable gate array development board implemented in a compact setup for fluorescence correlation spectroscopy.

作者信息

Kalinin Stanislav, Kühnemuth Ralf, Vardanyan Hayk, Seidel Claus A M

机构信息

Institut für Physikalische Chemie, Lehrstuhl für Molekulare Physikalische Chemie, Heinrich-Heine-Universität, Universitätsstrasse 1, Geb. 26.32.02, 40225 Düsseldorf, Germany.

出版信息

Rev Sci Instrum. 2012 Sep;83(9):096105. doi: 10.1063/1.4753994.

Abstract

We present a fast hardware photon correlator implemented in a field-programmable gate array (FPGA) combined with a compact confocal fluorescence setup. The correlator has two independent units with a time resolution of 4 ns while utilizing less than 15% of a low-end FPGA. The device directly accepts transistor-transistor logic (TTL) signals from two photon counting detectors and calculates two auto- or cross-correlation curves in real time. Test measurements demonstrate that the performance of our correlator is comparable with the current generation of commercial devices. The sensitivity of the optical setup is identical or even superior to current commercial devices. The FPGA design and the optical setup both allow for a straightforward extension to multi-color applications. This inexpensive and compact solution with a very good performance can serve as a versatile platform for uses in education, applied sciences, and basic research.

摘要

我们展示了一种在现场可编程门阵列(FPGA)中实现的快速硬件光子相关器,它与紧凑型共聚焦荧光装置相结合。该相关器有两个独立单元,时间分辨率为4纳秒,同时仅使用了低端FPGA不到15%的资源。该设备直接接受来自两个光子计数探测器的晶体管-晶体管逻辑(TTL)信号,并实时计算两条自相关或互相关曲线。测试测量表明,我们的相关器性能与当前一代商业设备相当。光学装置的灵敏度与当前商业设备相同甚至更高。FPGA设计和光学装置都允许直接扩展到多色应用。这种价格低廉、紧凑且性能出色的解决方案可作为教育、应用科学和基础研究中用途广泛的平台。

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