China-Japan Union Hospital, Jilin University, Changchun 130033, PR China.
Department of International Health, Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3B, DK-2200 Copenhagen, Denmark.
Microbiology (Reading). 2012 Dec;158(Pt 12):3014-3019. doi: 10.1099/mic.0.061192-0. Epub 2012 Sep 28.
Bacteria form complex surface-attached biofilm communities in nature. Biofilm cells differentiate into subpopulations which display tolerance towards antimicrobial agents. However, the signal transduction pathways regulating subpopulation differentiation in biofilms are largely unelucidated. In the present study, we show that the catabolite repression control protein Crc regulates the metabolic state of Pseudomonas aeruginosa cells in biofilms, and plays an important role in the development of antimicrobial-tolerant subpopulations in P. aeruginosa biofilms.
细菌在自然界中形成复杂的附着在表面的生物膜群落。生物膜中的细胞分化成亚群，这些亚群对抗菌剂表现出耐受性。然而，调节生物膜中亚群分化的信号转导途径在很大程度上尚未阐明。在本研究中，我们表明，分解代谢物阻遏控制蛋白 Crc 调节铜绿假单胞菌生物膜中细胞的代谢状态，并在铜绿假单胞菌生物膜中抗菌剂耐受亚群的发展中发挥重要作用。
