Department of Ophthalmology, Research Institute of Medical Sciences, Chonnam National University Medical School and Hospital, Gwangju, Korea.
Curr Eye Res. 2012 Dec;37(12):1084-90. doi: 10.3109/02713683.2012.717243. Epub 2012 Oct 1.
To evaluate the effect of umbilical cord serum (UCS) eye drops on corneal wound healing and haze in a mouse model of ocular chemical burn and compare with that of peripheral blood serum (PBS) eye drops or artificial tears (AT).
Chemical burn of the ocular surface was induced by 1N NaOH in C57BL/6 mice. Injured mice were topically treated with 20% UCS, 20% PBS, or AT four times daily. The changes of corneal epithelial defects and degree of haze were analyzed at 6 h, 1, 2, 3, 5, and 7 days, and histological examination was performed at 3 and 7 days. The concentration of IL-1β in the cornea was measured by enzyme-linked immunosorbent assay at 7 days after treatment.
The UCS group showed lower epithelial defect parameters compared with the PBS group at 1 and 2 days (p < 0.05), and with the AT group from 1-5 days (p < 0.05). The haze scores were significantly lower in the UCS group than in the PBS group at 2 and 3 days (p < 0.05), and in the AT group from 2-7 days (p < 0.05). Histological examination showed better epithelial integrity and lower stromal inflammation and edema in the UCS group than the other groups. IL-1β levels were 99.71 ± 85.22 and 230.76 ± 102.67 pg/ml in the UCS and PBS groups, respectively (p = 0.03).
UCS eye drops are more effective in improving corneal wound healing and reducing corneal haze compared with PBS eye drops and AT in experimental chemical burns.
评估脐带血清（UCS）滴眼液对眼部化学烧伤模型小鼠角膜伤口愈合和混浊的影响，并与外周血血清（PBS）滴眼液或人工泪液（AT）进行比较。
在 C57BL/6 小鼠的眼表面用 1N NaOH 诱导化学烧伤。受伤的小鼠每天用 20%UCS、20%PBS 或 AT 滴眼 4 次。在 6 h、1、2、3、5 和 7 天分析角膜上皮缺损和混浊程度的变化，并在 3 和 7 天进行组织学检查。在治疗后 7 天通过酶联免疫吸附试验测量角膜中 IL-1β的浓度。
与 PBS 组相比，UCS 组在 1 天和 2 天（p < 0.05），与 AT 组在 1-5 天（p < 0.05）时上皮缺损参数较低。与 PBS 组相比，UCS 组在 2 天和 3 天（p < 0.05），与 AT 组在 2-7 天（p < 0.05）时混浊评分较低。组织学检查显示，UCS 组上皮完整性更好，基质炎症和水肿程度更低。UCS 组和 PBS 组的 IL-1β 水平分别为 99.71 ± 85.22 和 230.76 ± 102.67 pg/ml（p = 0.03）。
与 PBS 滴眼液和 AT 相比，UCS 滴眼液在实验性化学烧伤中更有效地改善角膜伤口愈合和减少角膜混浊。
