Sætrom Pål
Department of Computer and Information Science, Norwegian University of Science and Technology, Trondheim, Norway.
Methods Mol Biol. 2013;942:169-77. doi: 10.1007/978-1-62703-119-6_9.
Short interfering RNAs (siRNAs) have become valued tools for knocking down specific genes. As such, siRNAs are routinely used to study gene function and are also being explored as therapeutic agents. Traditionally, siRNAs are designed to target one specific gene, but this chapter describes a procedure for designing dual-targeting siRNAs where the two strands in the siRNA duplex are both active and down-regulate different target genes through both siRNA and miRNA-like effects. The procedure can be used to create siRNAs that robustly target pairs of genes.
小干扰RNA（siRNAs）已成为用于敲低特定基因的重要工具。因此，siRNAs经常被用于研究基因功能，并且也正在作为治疗药物进行探索。传统上，siRNAs被设计用于靶向一个特定基因，但本章描述了一种设计双靶向siRNAs的方法，其中siRNA双链体中的两条链均具有活性，并通过siRNA和miRNA样效应下调不同的靶基因。该方法可用于创建能有效靶向基因对的siRNAs。
