Rocchiccioli Silvia, Andreassi Maria G, Cecchettini Antonella, Carpeggiani Clara, L'Abbate Antonio, Citti Lorenzo
Istitute of Clinical Physiology, University of Pisa, Pisa, Italy.
Coron Artery Dis. 2012 Nov;23(7):426-31. doi: 10.1097/MCA.0b013e328358781c.
Proteomics is considered a promising tool in the discovery of new biomarkers and/or therapeutic targets. The aim of this study was to identify proteins expressed in the plasma of survivors of myocardial infarction and possible correlations between expression of some proteins and the severity of coronary artery disease (CAD) in this population.
The study included 17 survivors (15 men; age=53±9 years) of myocardial infarction at young age (age<60 years) classified for the severity of CAD graded according to angiography and 10 healthy volunteers (nine men; age=54±9 years). Proteomic analysis was carried out using a high-throughput technology and MALDI TOF/TOF mass spectrometry.
Compared with the healthy population, 14 proteins were differentially expressed in patients, and were classified in three principal categories: contraction, inflammation, and coagulation. Results show a correlation between the angiographic severity, the extension of CAD, and the expression of some proteins. In particular decreased levels of vitamin D binding protein (VDBP) in the plasma of patients statistically correlated with the number of affected coronary arteries. Enzyme-linked immunosorbent assay test confirmed modulation of VDBP.
Our results suggest that some proteins are differentially expressed in atherosclerotic patients and their disregulation is strongly dependent on the severity of the artery disease. The down regulation of VDBP is confirmed and marked in multivessel disease patients.
蛋白质组学被认为是发现新生物标志物和/或治疗靶点的一种有前景的工具。本研究的目的是鉴定心肌梗死幸存者血浆中表达的蛋白质，以及该人群中某些蛋白质的表达与冠状动脉疾病（CAD）严重程度之间的可能相关性。
本研究纳入了17名年轻时（年龄<60岁）心肌梗死的幸存者（15名男性；年龄=53±9岁），根据血管造影对CAD严重程度进行分级，以及10名健康志愿者（9名男性；年龄=54±9岁）。使用高通量技术和基质辅助激光解吸电离飞行时间/飞行时间质谱进行蛋白质组学分析。
与健康人群相比，患者中有14种蛋白质差异表达，分为三个主要类别：收缩、炎症和凝血。结果显示血管造影严重程度、CAD范围与某些蛋白质的表达之间存在相关性。特别是患者血浆中维生素D结合蛋白（VDBP）水平降低与受影响冠状动脉的数量在统计学上相关。酶联免疫吸附试验证实了VDBP的调节。
我们的结果表明，一些蛋白质在动脉粥样硬化患者中差异表达，其失调强烈依赖于动脉疾病的严重程度。VDBP的下调在多支血管疾病患者中得到证实且较为明显。
