Boston University, Department of Biomedical Engineering, 44 Cummington Street, Boston, Massachusetts 02215, USA.
Opt Lett. 2012 Oct 1;37(19):4062-4. doi: 10.1364/OL.37.004062.
We present a technique to quantitatively image the phase of thin quasi-transparent samples using extended source incoherent illumination and off-axis detection apertures. Our technique is achromatic and polarization independent, requires no active elements, and can be readily adapted to standard bright-field microscopes. We demonstrate our technique by quantitatively reconstructing the phase of cheek cells and a microlens. The light efficient, single-shot nature of our technique enables phase imaging at frame rates that are camera limited.
我们提出了一种使用扩展光源非相干照明和离轴探测孔径对薄的近似透明样品的相位进行定量成像的技术。我们的技术具有消色差和偏振无关性,不需要主动元件,并且可以很容易地适应标准明场显微镜。我们通过定量重建颊细胞和微透镜的相位来演示我们的技术。我们的技术具有高效的单次拍摄特性,能够以相机限制的帧率进行相位成像。