Sun Jiawei, Wu Jiachen, Wu Song, Goswami Ruchi, Girardo Salvatore, Cao Liangcai, Guck Jochen, Koukourakis Nektarios, Czarske Juergen W
Laboratory of Measurement and Sensor System Technique (MST), TU Dresden, Helmholtzstrasse 18, 01069, Dresden, Germany.
Competence Center for Biomedical Computational Laser Systems (BIOLAS), TU Dresden, Dresden, Germany.
Light Sci Appl. 2022 Jul 5;11(1):204. doi: 10.1038/s41377-022-00898-2.
Quantitative phase imaging (QPI) is a label-free technique providing both morphology and quantitative biophysical information in biomedicine. However, applying such a powerful technique to in vivo pathological diagnosis remains challenging. Multi-core fiber bundles (MCFs) enable ultra-thin probes for in vivo imaging, but current MCF imaging techniques are limited to amplitude imaging modalities. We demonstrate a computational lensless microendoscope that uses an ultra-thin bare MCF to perform quantitative phase imaging with microscale lateral resolution and nanoscale axial sensitivity of the optical path length. The incident complex light field at the measurement side is precisely reconstructed from the far-field speckle pattern at the detection side, enabling digital refocusing in a multi-layer sample without any mechanical movement. The accuracy of the quantitative phase reconstruction is validated by imaging the phase target and hydrogel beads through the MCF. With the proposed imaging modality, three-dimensional imaging of human cancer cells is achieved through the ultra-thin fiber endoscope, promising widespread clinical applications.
定量相位成像（QPI）是一种无标记技术，可在生物医学中提供形态学和定量生物物理信息。然而，将这种强大的技术应用于体内病理诊断仍然具有挑战性。多芯光纤束（MCF）可实现用于体内成像的超薄探头，但目前的MCF成像技术仅限于幅度成像模式。我们展示了一种计算无透镜微型内窥镜，它使用超薄裸MCF以微米级横向分辨率和纳米级光程长度轴向灵敏度进行定量相位成像。测量侧的入射复光场从检测侧的远场散斑图案精确重建，无需任何机械移动即可在多层样品中进行数字重新聚焦。通过MCF对相位目标和水凝胶珠进行成像，验证了定量相位重建的准确性。利用所提出的成像模式，通过超薄光纤内窥镜实现了人类癌细胞的三维成像，有望在临床上广泛应用。
