Gomez-Flores R, Rodriguez-Padilla C, Tamez-Guerra R
Laboratorio de Immunología y Virología, Dr. Sergio Estrada Parra, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo Léon, México.
Cell Immunol. 1990 Mar;126(1):1-15. doi: 10.1016/0008-8749(90)90296-4.
Supernatant fluids (SNF) were obtained from peritoneal exudate adherent cells stimulated in vitro with sheep red blood cells (SRBC) or BCG, and SNF collected at 6 and 24 hr were able to induce the migratory responses of rat leukocytes from the spleen and peripheral blood. The production of these SNF was dependent on protein active synthesis upon in vitro antigenic stimulation. The chemotactic activity from 6-hr SNF was inhibited by using several proteolytic enzymes and temperatures. We found the macrophages to be the producer cell of this activity, while the T cells were the target cells. The chemotactic activity from 6-hr SNF was found not to be due to IL-1. Six-hour chemotactic activity has not been reported previously.
从用绵羊红细胞（SRBC）或卡介苗（BCG）体外刺激的腹膜渗出粘附细胞中获取上清液（SNF），在6小时和24小时收集的SNF能够诱导大鼠脾脏和外周血白细胞的迁移反应。这些SNF的产生依赖于体外抗原刺激后的蛋白质活性合成。使用几种蛋白水解酶和不同温度可抑制6小时SNF的趋化活性。我们发现巨噬细胞是这种活性的产生细胞，而T细胞是靶细胞。发现6小时SNF的趋化活性并非由白细胞介素-1引起。此前尚未报道过6小时的趋化活性。
