Department of Applied Chemistry, Anhui University of Technology, Maanshan, Anhui, 243002, People's Republic of China.
Luminescence. 2013 Sep-Oct;28(5):641-7. doi: 10.1002/bio.2409. Epub 2012 Oct 2.
A simple and green flow injection chemiluminescence (FI-CL) method for determination of the fungicide azoxystrobin was described for the first time. CL signal was generated when azoxystrobin was injected into a mixed stream of luminol and KMnO4 . The CL signal of azoxystrobin could be greatly improved when an off-line ultrasonic treatment was adopted. Meanwhile, the signal intensity increases with the analyte concentration proportionally. Several variables, such as the ultrasonic parameters, flow rate of reagents, concentrations of sodium hydroxide solution and CL reagents (potassium permanganate, luminol) were investigated, and the optimal CL conditions were obtained. Under optimal conditions, the linear range of 1-100 ng/mL for azoxystrobin was obtained and the detection limit (3σ) was determined as 0.13 ng/mL. The relative standard deviation was 1.5% for 10 consecutive measurements of 20 ng/mL azoxystrobin. The method has been applied to the determination of azoxystrobin residues in water samples.
首次描述了一种简单、绿色的流动注射化学发光（FI-CL）法来测定杀菌剂唑菌胺酯。当唑菌胺酯被注入到鲁米诺和高锰酸钾的混合流中时，会产生 CL 信号。采用离线超声处理可以大大提高唑菌胺酯的 CL 信号。同时，信号强度与分析物浓度成正比。考察了几种变量，如超声参数、试剂流速、氢氧化钠溶液和 CL 试剂（高锰酸钾、鲁米诺）的浓度，并获得了最佳的 CL 条件。在最佳条件下，获得了唑菌胺酯的线性范围为 1-100ng/mL，检测限（3σ）确定为 0.13ng/mL。对于连续 10 次测量 20ng/mL 唑菌胺酯，相对标准偏差为 1.5%。该方法已应用于水样中唑菌胺酯残留量的测定。
